College of Light Industry and Food, Zhongkai University of Agriculture and Engineering, Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology, Guangzhou 510225, China; Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; State Key Laboratory of Marine Food Processing & Safety Control, College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
College of Light Industry and Food, Zhongkai University of Agriculture and Engineering, Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology, Guangzhou 510225, China.
Food Res Int. 2024 Sep;192:114829. doi: 10.1016/j.foodres.2024.114829. Epub 2024 Jul 22.
This study focused on exploring the Zn chelating peptide GFLGSP: the characterization of structure/Zn chelating mode and the potential mechanisms for promoting Zn transport in Caco-2 cells. The findings revealed the bidentate chelating between Zn and carboxyl oxygen atom in Pro6 residue. Thereafter, the secondary structure of GFLGSP remained unchanged, but there was an increase in zeta potential and particle size. Notably, the GFLGSP-Zn complex enhanced the Zn transport rate and modulated ZIP4 and ZNT1 expression in a Caco-2 cells monolayer model. As revealed by molecular docking analysis, GFLGSP interacted with ZIP4 through intermolecular hydrogen bonds as well as Van der Waals forces. The Zn transport mechanisms of the GFLGSP-Zn complex encompassed ZIP4 (vital channel), endocytosis (primary pathway) and paracellular transport (supplementary pathway). Based on these results, the tilapia skin collagen-derived GFLGSP hold promise as the potential dietary Zn supplement.
本研究聚焦于探索 Zn 螯合肽 GFLGSP：结构/Zn 螯合模式的特征，以及在 Caco-2 细胞中促进 Zn 转运的潜在机制。研究结果揭示了 Zn 与 Pro6 残基中羧基氧原子的双齿螯合作用。随后，GFLGSP 的二级结构保持不变，但 ζ 电位和粒径增加。值得注意的是，GFLGSP-Zn 配合物提高了 Zn 的转运速率，并在 Caco-2 细胞单层模型中调节了 ZIP4 和 ZNT1 的表达。分子对接分析表明，GFLGSP 通过分子间氢键和范德华力与 ZIP4 相互作用。GFLGSP-Zn 配合物的 Zn 转运机制包括 ZIP4（重要通道）、内吞作用（主要途径）和细胞旁转运（补充途径）。基于这些结果，罗非鱼皮胶原蛋白衍生的 GFLGSP 有望成为潜在的膳食 Zn 补充剂。
