• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

新型乙醇溶性牡蛎肽-锌螯合剂的研究进展：结构特征、螯合机制及对 MEHP 诱导的睾丸间质细胞的潜在保护作用。

Novel Insights into Ethanol-Soluble Oyster Peptide-Zinc-Chelating Agents: Structural Characterization, Chelation Mechanism, and Potential Protection on MEHP-Induced Leydig Cells.

机构信息

Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, College of Food Science and Technology, National Research and Development Branch Center for Shellfish Processing, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Ocean University, Zhanjiang 524088, China.

School of Biological and Food Processing Engineering, Huanghuai University, Zhumadian 463000, China.

出版信息

Mar Drugs. 2024 Oct 10;22(10):465. doi: 10.3390/md22100465.

DOI:10.3390/md22100465
PMID:39452873
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11509544/
Abstract

Numerous studies have reported that mono-(2-ethylhexyl) phthalate (MEHP) (bioactive metabolite of Di(2-ethylhexyl) phthalate) has inhibitory effects on Leydig cells. This study aims to prepare an oyster peptide-zinc complex (PEP-Zn) to alleviate MEHP-induced damage in Leydig cells. Zinc-binding peptides were obtained through the following processes: zinc-immobilized affinity chromatography (IMAC-Zn), liquid chromatography-mass spectrometry technology (LC-MS/MS) analysis, molecular docking, molecular dynamic simulation, and structural characterization. Then, the Zn-binding peptide (PEP) named Glu-His-Ala-Pro-Asn-His-Asp-Asn-Pro-Gly-Asp-Leu (EHAPNHDNPGDL) was identified. EHAPNHDNPGDL showed the highest zinc-chelating ability of 49.74 ± 1.44%, which was higher than that of the ethanol-soluble oyster peptides (27.50 ± 0.41%). In the EHAPNHDNPGDL-Zn complex, Asn-5, Asp-7, Asn-8, His-2, and Asp-11 played an important role in binding to the zinc ion. Additionally, EHAPNHDNPGDL-Zn was found to increase the cell viability, significantly increase the relative activity of antioxidant enzymes and testosterone content, and decrease malondialdehyde (MDA) content in MEHP-induced TM3 cells. The results also indicated that EHAPNHDNPGDL-Zn could alleviate MEHP-induced apoptosis by reducing the protein level of p53, p21, and Bax, and increasing the protein level of Bcl-2. These results indicate that the zinc-chelating peptides derived from oyster peptides could be used as a potential dietary zinc supplement.

摘要

许多研究报告称，邻苯二甲酸二（2-乙基己基）酯（MEHP）（邻苯二甲酸二（2-乙基己基）酯的生物活性代谢物）对间质细胞具有抑制作用。本研究旨在制备牡蛎肽-锌复合物（PEP-Zn）以减轻 MEHP 诱导的间质细胞损伤。通过以下过程获得锌结合肽：锌固载亲和层析（IMAC-Zn）、液相色谱-质谱联用技术（LC-MS/MS）分析、分子对接、分子动力学模拟和结构表征。然后，鉴定出锌结合肽（PEP），命名为 Glu-His-Ala-Pro-Asn-His-Asp-Asn-Pro-Gly-Asp-Leu（EHAPNHDNPGDL）。EHAPNHDNPGDL 表现出最高的锌螯合能力，为 49.74±1.44%，高于乙醇可溶牡蛎肽（27.50±0.41%）。在 EHAPNHDNPGDL-Zn 复合物中，Asn-5、Asp-7、Asn-8、His-2 和 Asp-11 在与锌离子结合中起重要作用。此外，发现 EHAPNHDNPGDL-Zn 可通过降低 p53、p21 和 Bax 的蛋白水平，增加 Bcl-2 的蛋白水平，增加 TM3 细胞中细胞活力，显著增加抗氧化酶的相对活性和睾酮含量，降低丙二醛（MDA）含量，从而减轻 MEHP 诱导的细胞凋亡。结果还表明，EHAPNHDNPGDL-Zn 可以通过降低 p53、p21 和 Bax 的蛋白水平，增加 Bcl-2 的蛋白水平，减轻 MEHP 诱导的细胞凋亡。这些结果表明，牡蛎肽衍生的锌结合肽可作为一种潜在的膳食锌补充剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/570d/11509544/c3f7cfbfecba/marinedrugs-22-00465-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/570d/11509544/4586edf26235/marinedrugs-22-00465-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/570d/11509544/ec6615755561/marinedrugs-22-00465-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/570d/11509544/dfdc352cc856/marinedrugs-22-00465-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/570d/11509544/48db85fd63b9/marinedrugs-22-00465-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/570d/11509544/b8860aec7b2c/marinedrugs-22-00465-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/570d/11509544/3b1fade0fc73/marinedrugs-22-00465-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/570d/11509544/3c2794df84d6/marinedrugs-22-00465-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/570d/11509544/480c3801e2a4/marinedrugs-22-00465-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/570d/11509544/c3f7cfbfecba/marinedrugs-22-00465-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/570d/11509544/4586edf26235/marinedrugs-22-00465-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/570d/11509544/ec6615755561/marinedrugs-22-00465-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/570d/11509544/dfdc352cc856/marinedrugs-22-00465-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/570d/11509544/48db85fd63b9/marinedrugs-22-00465-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/570d/11509544/b8860aec7b2c/marinedrugs-22-00465-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/570d/11509544/3b1fade0fc73/marinedrugs-22-00465-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/570d/11509544/3c2794df84d6/marinedrugs-22-00465-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/570d/11509544/480c3801e2a4/marinedrugs-22-00465-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/570d/11509544/c3f7cfbfecba/marinedrugs-22-00465-g009.jpg

相似文献

1
Novel Insights into Ethanol-Soluble Oyster Peptide-Zinc-Chelating Agents: Structural Characterization, Chelation Mechanism, and Potential Protection on MEHP-Induced Leydig Cells.新型乙醇溶性牡蛎肽-锌螯合剂的研究进展：结构特征、螯合机制及对 MEHP 诱导的睾丸间质细胞的潜在保护作用。
Mar Drugs. 2024 Oct 10;22(10):465. doi: 10.3390/md22100465.
2
Molecular mechanisms mediating the effect of mono-(2-ethylhexyl) phthalate on hormone-stimulated steroidogenesis in MA-10 mouse tumor Leydig cells.介导邻苯二甲酸二（2-乙基己基）酯对 MA-10 小鼠肿瘤 Leydig 细胞激素刺激类固醇生成作用的分子机制。
Endocrinology. 2010 Jul;151(7):3348-62. doi: 10.1210/en.2010-0010. Epub 2010 May 12.
3
Oxidative stress and phthalate-induced down-regulation of steroidogenesis in MA-10 Leydig cells.氧化应激和邻苯二甲酸酯对 MA-10 间质细胞类固醇生成的下调作用。
Reprod Toxicol. 2013 Dec;42:95-101. doi: 10.1016/j.reprotox.2013.07.025. Epub 2013 Aug 19.
4
Effect of mono-ethylhexyl phthalate on MA-10 Leydig tumor cells.邻苯二甲酸单乙基己酯对MA-10睾丸间质细胞瘤细胞的影响。
Reprod Toxicol. 2001 Mar-Apr;15(2):171-87. doi: 10.1016/s0890-6238(01)00110-1.
5
Separation and identification of zinc-chelating peptides from sesame protein hydrolysate using IMAC-Zn²⁺ and LC-MS/MS.利用 IMAC-Zn²⁺ 和 LC-MS/MS 从芝麻蛋白水解物中分离和鉴定锌螯合肽。
Food Chem. 2012 Sep 15;134(2):1231-8. doi: 10.1016/j.foodchem.2012.02.204. Epub 2012 Mar 16.
6
Di-(2-ethylhexyl) phthalate exposure induces premature testicular senescence by disrupting mitochondrial respiratory chain through STAT5B-mitoSTAT3 in Leydig cell.邻苯二甲酸二（2-乙基己基）酯通过 STAT5B-mitoSTAT3 扰乱睾丸间质细胞中线粒体呼吸链诱导过早睾丸衰老。
Geroscience. 2024 Oct;46(5):4373-4396. doi: 10.1007/s11357-024-01119-x. Epub 2024 Mar 19.
7
Preparation and Characterization of an Oyster Peptide-Zinc Complex and Its Antiproliferative Activity on HepG Cells.牡蛎肽-锌配合物的制备及对 HepG 细胞的增殖抑制活性。
Mar Drugs. 2023 Oct 18;21(10):542. doi: 10.3390/md21100542.
8
Evaluation of cytotoxicity and oxidative DNA damaging effects of di(2-ethylhexyl)-phthalate (DEHP) and mono(2-ethylhexyl)-phthalate (MEHP) on MA-10 Leydig cells and protection by selenium.邻苯二甲酸二（2-乙基己基）酯（DEHP）和邻苯二甲酸单（2-乙基己基）酯（MEHP）对 MA-10 间质细胞的细胞毒性和氧化 DNA 损伤作用的评价及硒的保护作用。
Toxicol Appl Pharmacol. 2010 Oct 1;248(1):52-62. doi: 10.1016/j.taap.2010.07.016. Epub 2010 Jul 24.
9
Mono-(2-ethylhexyl) phthalate induces apoptosis through miR-16 in human first trimester placental cell line HTR-8/SVneo.邻苯二甲酸单（2-乙基己基）酯通过miR-16诱导人早孕胎盘细胞系HTR-8/SVneo凋亡。
Toxicol In Vitro. 2016 Mar;31:35-42. doi: 10.1016/j.tiv.2015.11.010. Epub 2015 Nov 17.
10
Mono(2-ethylhexyl) phthalate induces apoptosis in p53-silenced L02 cells via activation of both mitochondrial and death receptor pathways.邻苯二甲酸单（2-乙基己基）酯通过激活线粒体和死亡受体途径诱导p53沉默的L02细胞凋亡。
Environ Toxicol. 2015 Sep;30(10):1178-91. doi: 10.1002/tox.21990. Epub 2014 Apr 5.

本文引用的文献

1
Novel microbial fermentation for the preparation of iron-chelating scallop skirts peptides-its profile, identification, and possible binding mode.新型微生物发酵法制备铁螯合扇贝裙边肽的特性、鉴定及可能的结合模式。
Food Chem. 2024 Sep 1;451:139493. doi: 10.1016/j.foodchem.2024.139493. Epub 2024 Apr 29.
2
Oyster Peptide-Zinc Complex Ameliorates Di-(2-ethylhexyl) Phthalate-Induced Testis Injury in Male Mice and Improving Gut Microbiota.牡蛎肽锌复合物改善邻苯二甲酸二（2-乙基己基）酯诱导的雄性小鼠睾丸损伤并改善肠道微生物群。
Foods. 2023 Dec 27;13(1):93. doi: 10.3390/foods13010093.
3
ARTS is essential for di-2-ethylhexyl phthalate (DEHP)-induced apoptosis of mouse Leydig cells.
芳香化酶在邻苯二甲酸二（2-乙基己基）酯（DEHP）诱导的小鼠睾丸间质细胞凋亡中起关键作用。
Ecotoxicol Environ Saf. 2024 Jan 15;270:115882. doi: 10.1016/j.ecoenv.2023.115882. Epub 2024 Jan 2.
4
Cellular zinc metabolism and zinc signaling: from biological functions to diseases and therapeutic targets.细胞锌代谢和锌信号转导：从生物学功能到疾病和治疗靶点。
Signal Transduct Target Ther. 2024 Jan 3;9(1):6. doi: 10.1038/s41392-023-01679-y.
5
Insights into the interactions between etheric compounds and myofibrillar proteins using multi-spectroscopy, molecular docking, and molecular dynamics simulation.利用多谱学、分子对接和分子动力学模拟研究醚类化合物与肌原纤维蛋白的相互作用。
Food Res Int. 2024 Jan;175:113787. doi: 10.1016/j.foodres.2023.113787. Epub 2023 Dec 3.
6
Structure-Based In Silico Screening of Marine Phlorotannins for Potential Walrus Calicivirus Inhibitor.基于结构的海洋岩藻黄烷醇类化合物抗海象杯状病毒抑制剂的计算机筛选
Int J Mol Sci. 2023 Oct 30;24(21):15774. doi: 10.3390/ijms242115774.
7
Comparison of Neuroprotection and Regulating Properties on Gut Microbiota between Selenopeptide Val-Pro-Arg-Lys-Leu-SeMet and Its Native Peptide Val-Pro-Arg-Lys-Leu-Met In Vitro and In Vivo.硒代肽 Val-Pro-Arg-Lys-Leu-SeMet 及其天然肽 Val-Pro-Arg-Lys-Leu-Met 在体外和体内的神经保护和调节肠道微生物群比较。
J Agric Food Chem. 2023 Aug 16;71(32):12203-12215. doi: 10.1021/acs.jafc.3c02918. Epub 2023 Aug 2.
8
Metal-binding peptides and their potential to enhance the absorption and bioavailability of minerals.金属结合肽及其增强矿物质吸收和生物利用度的潜力。
Food Chem. 2023 Dec 1;428:136678. doi: 10.1016/j.foodchem.2023.136678. Epub 2023 Jun 22.
9
Screening and Evaluation of Novel α-Glucosidase Inhibitory Peptides from Seed Cake Based on Molecular Docking Combined with Molecular Dynamics Simulation.基于分子对接结合分子动力学模拟筛选和评价菜籽饼中新型α-葡萄糖苷酶抑制剂肽。
J Agric Food Chem. 2023 Jul 12;71(27):10326-10337. doi: 10.1021/acs.jafc.3c00826. Epub 2023 Jun 8.
10
Computer-Aided Design of Lasso-like Self-Assembling Anticancer Peptides with Multiple Functions for Targeted Self-Delivery and Cancer Treatments.基于计算机辅助设计的多功能套索样自组装抗癌肽用于靶向自递药和癌症治疗
ACS Nano. 2022 Sep 27;16(9):13783-13799. doi: 10.1021/acsnano.2c01014. Epub 2022 Sep 13.