Investigador por México-Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Mexico.
Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Mexico.
Proteomics. 2024 Aug;24(16):e2400117. doi: 10.1002/pmic.202400117.
Inferring evolutionary relationships among organisms has been a fundamental problem in evolutionary biology. The current phylogenetic molecular methods serve as the baseline model to test new evolutionary hypotheses with taxonomic purposes. Leishmaniinae trypanosomatids subfamily includes protozoan parasites of clinical importance to humans. They have an intricate taxonomic history defined by morphological elements, host range, and molecular phylogenies. Unraveling the increasing diversity of this group has shown limitations in reconstructing the true evolutionary relationships among Trypanosomatidae species. Toward the goal of inferring evolutionary relationships that help to resolve phylogenetic and taxonomic controversies among parasites of the subfamily Leishmaniinae, Mule et al. propose the method PhyloQuant as a valuable approach, based on differential protein expression obtained from high throughput mass spectrometry data. Employing a pioneering methodological approach, Mule et al. assess the taxonomic problem for species hypothesis within Leishmaniinae, from quantitative phenetic protein expression profiles, in contrast to the standard multilocus phylogenetic approaches.
推断生物之间的进化关系一直是进化生物学中的一个基本问题。目前的系统发育分子方法是测试具有分类学目的的新进化假设的基准模型。Leishmaniinae 原生动物亚科包括对人类具有临床重要性的原生动物寄生虫。它们具有复杂的分类历史，由形态元素、宿主范围和分子系统发育定义。揭示该组日益增加的多样性表明，在重建真核生物门物种之间的真正进化关系方面存在局限性。为了推断有助于解决 Leishmaniinae 亚科寄生虫之间的系统发育和分类争议的进化关系，Mule 等人提出了 PhyloQuant 方法，该方法基于从高通量质谱数据中获得的差异蛋白表达。Mule 等人采用开创性的方法学方法，从定量表型蛋白表达谱评估 Leishmaniinae 内物种假设的分类问题，与标准的多位点系统发育方法形成对比。
