Mule Simon Ngao, Alemán Evaristo Villalba, Rosa-Fernandes Livia, Saad Joyce S, de Oliveira Gilberto Santos, Martins Deivid, Angeli Claudia Blanes, Brandt-Almeida Deborah, Cortez Mauro, Larsen Martin Røssel, Shaw Jeffrey J, Teixeira Marta M G, Palmisano Giuseppe
Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, DK, Denmark.
Proteomics. 2024 Sep;24(18):e2100313. doi: 10.1002/pmic.202100313. Epub 2024 Jun 8.
Evolutionary relationships among parasites of the subfamily Leishmaniinae, which comprises pathogen agents of leishmaniasis, were inferred based on differential protein expression profiles from mass spectrometry-based quantitative data using the PhyloQuant method. Evolutionary distances following identification and quantification of protein and peptide abundances using Proteome Discoverer and MaxQuant software were estimated for 11 species from six Leishmaniinae genera. Results clustered all dixenous species of the genus Leishmania, subgenera L. (Leishmania), L. (Viannia), and L. (Mundinia), sister to the dixenous species of genera Endotrypanum and Porcisia. Placed basal to the assemblage formed by all these parasites were the species of genera Zelonia, Crithidia, and Leptomonas, so far described as monoxenous of insects although eventually reported from humans. Inferences based on protein expression profiles were congruent with currently established phylogeny using DNA sequences. Our results reinforce PhyloQuant as a valuable approach to infer evolutionary relationships within Leishmaniinae, which is comprised of very tightly related trypanosomatids that are just beginning to be phylogenetically unraveled. In addition to evolutionary history, mapping of species-specific protein expression is paramount to understand differences in infection processes, tissue tropisms, potential to jump from insects to vertebrates including humans, and targets for species-specific diagnostic and drug development.
利什曼原虫亚科的寄生虫是利什曼病的病原体，基于使用PhyloQuant方法从基于质谱的定量数据中获得的差异蛋白质表达谱，推断了它们之间的进化关系。使用Proteome Discoverer和MaxQuant软件对蛋白质和肽丰度进行鉴定和定量后，估计了来自六个利什曼原虫亚科属的11个物种的进化距离。结果将利什曼原虫属的所有双宿主物种，即利什曼原虫亚属（利什曼原虫）、利什曼原虫亚属（维氏亚属）和利什曼原虫亚属（蒙氏亚属）聚类在一起，它们是锥虫属和猪锥虫属双宿主物种的姐妹群。在所有这些寄生虫形成的组合的基部是泽洛尼亚属、短膜虫属和细滴虫属的物种，尽管最终在人类中被报道，但迄今为止被描述为昆虫的单宿主寄生虫。基于蛋白质表达谱的推断与目前使用DNA序列建立的系统发育一致。我们的结果强化了PhyloQuant作为推断利什曼原虫亚科内进化关系的一种有价值方法的地位，利什曼原虫亚科由关系非常密切的锥虫组成，它们的系统发育刚刚开始被揭示。除了进化历史外，绘制物种特异性蛋白质表达图谱对于理解感染过程、组织嗜性、从昆虫跳到包括人类在内的脊椎动物的潜力以及物种特异性诊断和药物开发的靶点的差异至关重要。
