Bagherani Nooshin, Ghanadan Alireza, Mirmomeni Golshan, Firooz Alireza, Smoller Bruce R, Shojaei Reza, Rafipour Haniyeh, Bagherani Negin, Abdolhosseini Mansoreh, Tavoosidana Gholamreza
Dr. Bagherani is with the Department of Molecular Medicine, School of Advanced Technologies in Medicine at the Tehran University of Medical Sciences in Tehran, Iran.
Dr. Ghanadan is with the Department of Dermatopathology, Razi Hospital and Department of Pathology, Cancer Institute at the Imam Khoemini Hospital Complex in Tehran, Iran.
J Clin Aesthet Dermatol. 2024 Aug;17(8):62-69.
Skin aging as a continuous and irreversible process is mainly the result of alterations of function and structure of the dermis. Among the modalities used for treating skin aging, carboxytherapy has been introduced as a safe minimally-invasive method for rejuvenation, reparation, and reconditioning of the skin.
We assessed the efficacy of carboxytherapy for the treatment of intrinsic skin aging through pathological and immunohistochemical (IHC) investigations.
Our study was a split-body, randomized clinical trial on 15 female patients with intrinsic skin aging of the abdomen. Carboxytherapy was performed on one side of the abdomen, weekly for 10 sessions, while the other side was left untreated. Two weeks after the last session, skin biopsies were taken from both sides of the abdomen. Staining with hematoxylin-eosin, Masson-trichrome, and Orcein Giemsa was performed for the assessment of epidermal and dermal thickness, collagen, and elastin organization, respectively. IHC examination was performed for investigation of TGF-β1 and VEGF.
Pathological examination showed a significant increase in epidermal and dermal thickness and re-organization of collagens and elastic fibers with carboxytherapy. IHC examinations revealed a significantly increased expression of TGF-β1 and VEGF with carboxytherapy.
Our study demonstrated the effectiveness of carboxytherapy in treating and reversing intrinsic aging skin through pathological and IHC studies.
皮肤老化是一个持续且不可逆的过程，主要是真皮功能和结构改变的结果。在用于治疗皮肤老化的方法中，羧基疗法已被引入作为一种安全的微创皮肤年轻化、修复和调理方法。
我们通过病理和免疫组织化学（IHC）研究评估羧基疗法治疗皮肤自然老化的疗效。
我们的研究是一项对15名腹部皮肤自然老化女性患者进行的分体随机临床试验。在腹部一侧进行羧基疗法，每周一次，共10次，而另一侧不进行治疗。最后一次治疗后两周，从腹部两侧取皮肤活检组织。分别用苏木精-伊红、Masson三色染色和地衣红吉姆萨染色评估表皮和真皮厚度、胶原蛋白和弹性蛋白组织。进行IHC检查以研究转化生长因子-β1（TGF-β1）和血管内皮生长因子（VEGF）。
病理检查显示，羧基疗法可使表皮和真皮厚度显著增加，胶原蛋白和弹性纤维重新组织。IHC检查显示，羧基疗法可使TGF-β1和VEGF的表达显著增加。
我们的研究通过病理和IHC研究证明了羧基疗法在治疗和逆转皮肤自然老化方面的有效性。
