[Studies on staphylococcal enterotoxin B. III. Purification with absorbents].
作者信息
Chen C P, Wang N L, Tai F H, Chung J S
出版信息
Zhonghua Min Guo Wei Sheng Wu Xue Za Zhi. 1979 Jun;12(2):65-9.
PMID:391509
Abstract
The biological and immunological activities of Staphylococcal enterotoxin B are stable in pH 2.0 approximately 11.0, and also resistant to proteolytic enzyme (trypsin, pepsin) digestion for 3 approximate 4 hours. Therefore, the toxin could be purified with trypsin digest and then absorbed on kaolin and Kieselgel, followed by eluting the different pH buffer solutions. Further purifications was chromatographied on CM-Sephadex column.
摘要
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