Reiser R F, Robbins R N, Noleto A L, Khoe G P, Bergdoll M S
Infect Immun. 1984 Sep;45(3):625-30. doi: 10.1128/iai.45.3.625-630.1984.
A third staphylococcal enterotoxin C (C3) has been identified, purified, and characterized. Staphylococcal enterotoxin C3 was identified from a Staphylococcus aureus isolated received from England. The purified toxin was determined by gel permeation chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis to be a simple protein with a molecular weight of 26,900. The isoelectric point of the major band was determined by isoelectric focusing in polyacrylamide gels to be 8.15. The reaction of enterotoxin C3 with its specific antibody was not affected by tryptic digestion at pH 8.0 or peptic digestion at pH 4.5. The enterotoxin C3 consisted of 236 amino acid residues. Serine was shown to be the NH2-terminal amino acid residue by end group analysis. The protein was highly emetic in cynomolgus monkeys both per os and intravenously.
已鉴定、纯化并表征了第三种葡萄球菌肠毒素C（C3）。葡萄球菌肠毒素C3是从一株来自英国的金黄色葡萄球菌分离物中鉴定出来的。通过凝胶渗透色谱法和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳确定纯化后的毒素是一种分子量为26,900的简单蛋白质。通过在聚丙烯酰胺凝胶中进行等电聚焦测定，主要条带的等电点为8.15。肠毒素C3与其特异性抗体的反应不受pH 8.0条件下的胰蛋白酶消化或pH 4.5条件下的胃蛋白酶消化的影响。肠毒素C3由236个氨基酸残基组成。通过末端基团分析表明丝氨酸是NH2末端氨基酸残基。该蛋白质经口和静脉注射对食蟹猴均具有高度致吐性。
