葡萄球菌肠毒素C3的鉴定、纯化及某些理化性质
Identification, purification, and some physicochemical properties of staphylococcal enterotoxin C3.
作者信息
Reiser R F, Robbins R N, Noleto A L, Khoe G P, Bergdoll M S
出版信息
Infect Immun. 1984 Sep;45(3):625-30. doi: 10.1128/iai.45.3.625-630.1984.
Abstract
A third staphylococcal enterotoxin C (C3) has been identified, purified, and characterized. Staphylococcal enterotoxin C3 was identified from a Staphylococcus aureus isolated received from England. The purified toxin was determined by gel permeation chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis to be a simple protein with a molecular weight of 26,900. The isoelectric point of the major band was determined by isoelectric focusing in polyacrylamide gels to be 8.15. The reaction of enterotoxin C3 with its specific antibody was not affected by tryptic digestion at pH 8.0 or peptic digestion at pH 4.5. The enterotoxin C3 consisted of 236 amino acid residues. Serine was shown to be the NH2-terminal amino acid residue by end group analysis. The protein was highly emetic in cynomolgus monkeys both per os and intravenously.
摘要
已鉴定、纯化并表征了第三种葡萄球菌肠毒素C（C3）。葡萄球菌肠毒素C3是从一株来自英国的金黄色葡萄球菌分离物中鉴定出来的。通过凝胶渗透色谱法和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳确定纯化后的毒素是一种分子量为26,900的简单蛋白质。通过在聚丙烯酰胺凝胶中进行等电聚焦测定，主要条带的等电点为8.15。肠毒素C3与其特异性抗体的反应不受pH 8.0条件下的胰蛋白酶消化或pH 4.5条件下的胃蛋白酶消化的影响。肠毒素C3由236个氨基酸残基组成。通过末端基团分析表明丝氨酸是NH2末端氨基酸残基。该蛋白质经口和静脉注射对食蟹猴均具有高度致吐性。
相似文献
1
Identification, purification, and some physicochemical properties of staphylococcal enterotoxin C3.葡萄球菌肠毒素C3的鉴定、纯化及某些理化性质
Infect Immun. 1984 Sep;45(3):625-30. doi: 10.1128/iai.45.3.625-630.1984.
2
Purification and some physicochemical properties of toxic-shock toxin.中毒性休克毒素的纯化及某些物理化学性质
Biochemistry. 1983 Aug 2;22(16):3907-12. doi: 10.1021/bi00285a028.
3
Purification and some physicochemical properties of staphylococcal enterotoxin D.
Biochemistry. 1979 May 15;18(10):1937-42. doi: 10.1021/bi00577a013.
4
The application of QAE-Sephadex for the purification of two staphylococcal enterotoxins. I. Purification of enterotoxin C2.QAE-葡聚糖在两种葡萄球菌肠毒素纯化中的应用。I. 肠毒素C2的纯化。
Biochim Biophys Acta. 1975 May 30;393(1):148-58. doi: 10.1016/0005-2795(75)90225-1.
5
Identification and purification of a new staphylococcal enterotoxin, H.一种新型葡萄球菌肠毒素H的鉴定与纯化
Appl Environ Microbiol. 1995 Apr;61(4):1438-43. doi: 10.1128/aem.61.4.1438-1443.1995.
6
A simple purification method for enterotoxin F produced by Staphylococcus aureus and some properties of the toxin.一种用于金黄色葡萄球菌产生的肠毒素F的简单纯化方法及该毒素的一些特性。
Antonie Van Leeuwenhoek. 1982 Dec;48(5):447-55. doi: 10.1007/BF00448416.
7
Chromatofocusing: a new method for purification of staphylococcal enterotoxins B and C1.层析聚焦法：一种纯化葡萄球菌肠毒素B和C1的新方法。
Appl Environ Microbiol. 1983 Dec;46(6):1323-30. doi: 10.1128/aem.46.6.1323-1330.1983.
8
Purification of staphylococcal enterotoxin E.
Nat Toxins. 1993;1(4):250-4. doi: 10.1002/nt.2620010409.
9
Novel method for purification of staphylococcal enterotoxin A.纯化葡萄球菌肠毒素A的新方法。
Appl Environ Microbiol. 1988 Jul;54(7):1761-5. doi: 10.1128/aem.54.7.1761-1765.1988.
10
The application of QAE-Sephadex for the purification of two staphylococcal enterotoxins. II. Purification of enterotoxin A.
Biochim Biophys Acta. 1975 May 30;393(1):159-64. doi: 10.1016/0005-2795(75)90226-3.
引用本文的文献
1
Staphylococcal Enterotoxin C-An Update on SEC Variants, Their Structure and Properties, and Their Role in Foodborne Intoxications.葡萄球菌肠毒素C——SEC变体、其结构与特性及其在食源性中毒中作用的最新进展
Toxins (Basel). 2020 Sep 10;12(9):584. doi: 10.3390/toxins12090584.
2
Basis of Virulence in Enterotoxin-Mediated Staphylococcal Food Poisoning.肠毒素介导的葡萄球菌食物中毒的毒力基础
Front Microbiol. 2018 Mar 13;9:436. doi: 10.3389/fmicb.2018.00436. eCollection 2018.
3
Characterization of Human Type C Enterotoxin Produced by Clinical S. epidermidis Isolates.临床表皮葡萄球菌分离株产生的人 C 型肠毒素的特性。
Toxins (Basel). 2018 Mar 27;10(4):139. doi: 10.3390/toxins10040139.
4
Staphylococcal enterotoxin C in synovial fluid of patients with rheumatoid arthritis.类风湿性关节炎患者滑液中的葡萄球菌肠毒素C
Iran Red Crescent Med J. 2014 Oct 5;16(10):e16075. doi: 10.5812/ircmj.16075. eCollection 2014 Oct.
5
Inhibition of staphylococcal enterotoxin B-induced lymphocyte proliferation and tumor necrosis factor alpha secretion by MAb5, an anti-toxic shock syndrome toxin 1 monoclonal antibody.抗中毒性休克综合征毒素1单克隆抗体MAb5对葡萄球菌肠毒素B诱导的淋巴细胞增殖及肿瘤坏死因子α分泌的抑制作用
Infect Immun. 2000 Jun;68(6):3261-8. doi: 10.1128/IAI.68.6.3261-3268.2000.
6
Rapid and specific detection of toxigenic Staphylococcus aureus: use of two multiplex PCR enzyme immunoassays for amplification and hybridization of staphylococcal enterotoxin genes, exfoliative toxin genes, and toxic shock syndrome toxin 1 gene.产毒金黄色葡萄球菌的快速特异性检测：使用两种多重PCR酶免疫分析法对葡萄球菌肠毒素基因、剥脱毒素基因和中毒性休克综合征毒素1基因进行扩增和杂交。
J Clin Microbiol. 1998 Sep;36(9):2548-53. doi: 10.1128/JCM.36.9.2548-2553.1998.
7
Staphylococcal food poisoning in the United Kingdom, 1969-90.1969 - 1990年英国的葡萄球菌食物中毒
Epidemiol Infect. 1993 Jun;110(3):519-31. doi: 10.1017/s0950268800050949.
8
Characterization of novel type C staphylococcal enterotoxins: biological and evolutionary implications.新型C型葡萄球菌肠毒素的特性：生物学及进化意义
Infect Immun. 1993 Oct;61(10):4254-62. doi: 10.1128/iai.61.10.4254-4262.1993.
9
Identification and purification of a new staphylococcal enterotoxin, H.一种新型葡萄球菌肠毒素H的鉴定与纯化
Appl Environ Microbiol. 1995 Apr;61(4):1438-43. doi: 10.1128/aem.61.4.1438-1443.1995.
10
Purification and characterization of an extracellular cytolysin produced by Vibrio vulnificus.创伤弧菌产生的一种细胞外溶素的纯化与特性分析
Infect Immun. 1985 Apr;48(1):62-72. doi: 10.1128/iai.48.1.62-72.1985.
本文引用的文献
1
The free amino groups of insulin.胰岛素的游离氨基
Biochem J. 1945;39(5):507-15. doi: 10.1042/bj0390507.
2
Production of enterotoxin a.肠毒素A的产生
Appl Microbiol. 1966 Nov;14(6):966-72. doi: 10.1128/am.14.6.966-972.1966.
3
Disk electrophoresis of basic proteins and peptides on polyacrylamide gels.碱性蛋白质和肽在聚丙烯酰胺凝胶上的圆盘电泳。
Nature. 1962 Jul 21;195:281-3. doi: 10.1038/195281a0.
4
Recent developments in techniques for terminal and sequence studies in peptides and proteins.肽和蛋白质末端及序列研究技术的最新进展。
Methods Biochem Anal. 1955;2:359-425. doi: 10.1002/9780470110188.ch12.
5
Some observations on the assay of staphylococcal enterotoxin by the monkey-feeding test.关于用猴喂养试验测定葡萄球菌肠毒素的一些观察
J Lab Clin Med. 1953 May;41(5):782-8.
6
Isolation of specific and common antibodies to staphylococcal enterotoxins B, C1, and C2.针对葡萄球菌肠毒素B、C1和C2的特异性及共同抗体的分离
Infect Immun. 1980 Feb;27(2):431-4. doi: 10.1128/iai.27.2.431-434.1980.
7
Immunoautoradiographic detection of proteins after electrophoretic transfer from gels to diazo-paper: analysis of adenovirus encoded proteins.蛋白质从凝胶电泳转移至重氮纸后的免疫放射自显影检测：腺病毒编码蛋白的分析
Proc Natl Acad Sci U S A. 1981 Jan;78(1):177-81. doi: 10.1073/pnas.78.1.177.
8
Purification and some physicochemical properties of toxic-shock toxin.中毒性休克毒素的纯化及某些物理化学性质
Biochemistry. 1983 Aug 2;22(16):3907-12. doi: 10.1021/bi00285a028.
9
Monoclonal antibodies to staphylococcal enterotoxins B and C: cross-reactivity and localization of epitopes on tryptic fragments.抗葡萄球菌肠毒素B和C的单克隆抗体：表位在胰蛋白酶消化片段上的交叉反应性及定位
Infect Immun. 1984 Jul;45(1):281-5. doi: 10.1128/iai.45.1.281-285.1984.
10
The complete amino acid sequence of staphylococcal enterotoxin C1.
J Biol Chem. 1983 May 25;258(10):6300-6.
Zotero 插件