Cainarca M, Casella P, Mauri A
Quad Sclavo Diagn. 1985 Sep;21(3):274-9.
A study was carried out on 150 strains of staphylococci of human origin in order to evaluate the possibility of achieving identification that is accurate, swift and perhaps economical, with the following aims in mind: a) the testing of fast systems for the detection of coagulase of Staphyslide and Sero Stat compared with classical coagulase in a test tube with EDTA and citrate; b) the evaluation of the API Staph system by comparing identification obtained through subculturing the strains in agar P, as suggested by manufacturers, with those that have been streaked directly with material on triptose agar. All the experiments were carried out in both laboratories and in double blindness. The strains of Staphylococcus aureus were 88, 90 and 98% respectively, identified by the Staphyslide, Sero Stat and coagulase in a test tube. The same results were obtained by greatly reducing the amount of the kits reactive substance. The facts show that system can take place in the microbiological laboratory to improve screening processes. The API Staph system identified 96% of all the strains and 89% of negative coagulase. The methods used to identify through primary culture in triptose are mirrored with subculture from agar P. Using this medium to streak urine, one can expect to prepare the recognition of the isolated strains the day before.
对150株源自人类的葡萄球菌进行了一项研究，目的是评估实现准确、快速且可能经济的鉴定的可能性，具体目标如下：a）将用于检测葡萄球菌凝固酶的快速系统Staphyslide和Sero Stat与在含有乙二胺四乙酸（EDTA）和柠檬酸盐的试管中的经典凝固酶检测方法进行比较；b）通过按照制造商建议将菌株在琼脂P上进行传代培养所获得的鉴定结果，与直接用材料在胰蛋白胨琼脂上划线培养所获得的鉴定结果相比较，来评估API葡萄球菌系统。所有实验均在两个实验室进行，且采用双盲方式。金黄色葡萄球菌菌株分别通过Staphyslide、Sero Stat和试管凝固酶鉴定，鉴定率分别为88%、90%和98%。通过大幅减少试剂盒反应物质的用量也获得了相同结果。事实表明，该系统可在微生物实验室中用于改进筛选过程。API葡萄球菌系统鉴定出了所有菌株的96%以及凝固酶阴性菌株的89%。通过在胰蛋白胨中进行原代培养来鉴定的方法与从琼脂P传代培养的方法相似。使用这种培养基对尿液进行划线培养，有望在前一天就对分离出的菌株进行识别。
