Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, China.
Department of Genetics, Wuhan University, Wuhan, Hubei 430071, China; School of Life Science and Technology, Harbin Institute of Technology, Harbin 150080, China.
Sci Total Environ. 2024 Nov 15;951:175512. doi: 10.1016/j.scitotenv.2024.175512. Epub 2024 Aug 14.
Prometryn is commonly used in agricultural and non-agricultural settings. However, possible harm to aquatic organisms remains a persistent concern. Prometryn was also the only one of the 26 triazine herbicides detected in this study. Numerous studies have assessed the harmful effects of prometryn in teleost fish and shrimp. There is a lack of information regarding the ecological and human health risks, as well as the toxic mechanisms affecting crayfish. In this study, human health risk assessment (THQ) and ecological risk assessment (RQ) were conducted on P. clarkii in the rice-crayfish co-culture (IRCC) farming model. The 96 h of exposure to 0.286 mg/L and 1.43 mg/L prometryn was conducted to investigate the potential effects and molecular mechanisms of hepatopancreatic resistance to prometryn in P. clarkii. The original sample analysis revealed that the THQ calculated from the prometryn levels in the muscle and hepatopancreas was below 0.1, suggesting no threat to human health. However, the calculated RQ values were >0.1, indicating a risk to P. clarkii. Histological analysis and biochemical index detection of the experimental samples revealed that the hepatopancreatic injury and oxidative damage in P. clarkii were caused by prometryn. Moreover, transcriptome analysis identified 2512 differentially expressed genes (DEGs) after 96 h of prometryn exposure. Prometryn exposure caused significant changes in metabolic pathways, including oxoacid metabolic processes and cytochrome P450-associated drug metabolism. Further hub gene analysis via PPI indicated that exposure to prometryn may inhibit lipid synthesis, storage, and amino acid transport and affect glucose metabolic pathways and hormone synthesis. Additionally, we hypothesized that prometryn-triggered cell death could be linked to the PI3K-Akt signaling cascade. This study's findings have significant meaning for the efficient and logical application of herbicides in IRCC, ultimately aiding in advancing a highly productive agricultural system.
扑灭津常用于农业和非农业领域。然而，对水生生物可能造成的危害仍然是一个持续存在的问题。在本研究中，扑灭津也是检测到的 26 种三嗪类除草剂中唯一的一种。大量研究评估了扑灭津对硬骨鱼类和虾类的有害影响。然而，有关其对克氏原螯虾的生态和人类健康风险，以及影响其毒性的机制的信息还很缺乏。在这项研究中，我们对稻-虾共作（IRCC）养殖模式中的克氏原螯虾进行了人类健康风险评估（THQ）和生态风险评估（RQ）。我们进行了 96 小时的 0.286mg/L 和 1.43mg/L 扑灭津暴露实验，以研究扑灭津对克氏原螯虾肝胰腺的潜在影响和分子机制。原始样本分析表明，从肌肉和肝胰腺中扑灭津水平计算得出的 THQ 低于 0.1，这表明对人类健康没有威胁。然而，计算出的 RQ 值大于 0.1，这表明扑灭津对克氏原螯虾存在风险。实验样本的组织学分析和生化指标检测表明，扑灭津导致了克氏原螯虾的肝胰腺损伤和氧化损伤。此外，转录组分析表明，在 96 小时的扑灭津暴露后，有 2512 个差异表达基因（DEGs）。扑灭津暴露导致代谢途径发生显著变化，包括氧化酸代谢过程和细胞色素 P450 相关的药物代谢。通过 PPI 进行的进一步枢纽基因分析表明，扑灭津暴露可能会抑制脂质的合成、储存和氨基酸的转运，并影响葡萄糖代谢途径和激素的合成。此外，我们假设扑灭津引发的细胞死亡可能与 PI3K-Akt 信号级联有关。这项研究的结果对于在 IRCC 中高效、合理地应用除草剂具有重要意义，最终有助于推进高产农业系统的发展。
