Department of Biochemistry, AP-HP, Necker-Enfants Malades Hospital, Paris, France.
Department of Biochemistry, AP-HP, Necker-Enfants Malades Hospital, Paris, France; University of Paris Cité, INSERM, Innovative Therapies in Haemostasis, Paris, France.
Clin Chim Acta. 2025 Jan 1;564:119925. doi: 10.1016/j.cca.2024.119925. Epub 2024 Aug 14.
In pediatric cardiology, the fact that some new biomarkers have assay-specific normal values has to be considered for correct clinical decisions. The current study aimed to provide age-adjusted normative values for NT-proBNP and Galectin-3 using the Abbott immunoassay system from a prospective French pediatric cohort sera collection and to validate our data for NT-proBNP on a second retrospective cohort.
We analyzed 283 consecutive samples for NT-proBNP and 140 samples for Galectin-3 collected from apparently healthy children (0-18 years) with outpatient treatment at our institution (Hôpital Necker-Enfants malades, Paris, France) during 24 months.
For NT-proBNP and Galectin-3, we establish four age partitions, respectively two (<2 years / >2 years) and establish upper reference values and their 90 % CI for each biomarker (Galectin-3 (ng/mL): 56 [44-70] / 26 [23-29]). We evaluated the diagnostic performance of our upper reference values of NT-proBNP on a retrospective cohort (n = 428) with positive predictive value of 0.92.
Using Abbott immunoassay system, we report age-specific reference values for NT-proBNP and for the first time for Galectin-3 in a healthy French pediatric cohort. These data call for larger cohort studies to define more robustly percentiles and diagnostic performance for NT-proBNP.
在儿科心脏病学中，一些新的生物标志物具有特定于检测方法的正常值，这在做出正确的临床决策时必须考虑到。本研究旨在使用 Abbott 免疫分析系统从前瞻性法国儿科队列的血清中提供 NT-proBNP 和半乳糖凝集素-3 的年龄校正参考值，并验证我们在第二个回顾性队列中对 NT-proBNP 数据的验证。
我们分析了来自在我们机构（巴黎 Necker-Enfants malades 医院，法国）接受门诊治疗的 283 例连续样本的 NT-proBNP 和 140 例 Galectin-3 样本，这些样本来自于显然健康的儿童（0-18 岁）。
对于 NT-proBNP 和 Galectin-3，我们分别建立了四个年龄分区，即两个（<2 岁/>2 岁），并为每个生物标志物建立了上参考值及其 90%CI（Galectin-3（ng/mL）：56 [44-70]/26 [23-29]）。我们评估了 NT-proBNP 上参考值在回顾性队列（n=428）中的诊断性能，阳性预测值为 0.92。
使用 Abbott 免疫分析系统，我们报告了特定于年龄的 NT-proBNP 参考值，并且首次在健康的法国儿科队列中报告了 Galectin-3 的参考值。这些数据呼吁进行更大规模的队列研究，以更准确地确定 NT-proBNP 的百分位数和诊断性能。
