Life Sciences Division, Institute of Advanced Study in Science and Technology, Paschim Boragaon, Guwahati, 781035, Assam, India; Chemical Biology Laboratory II, Life Science Division, Institute of Advanced Study in Science and Technology, Paschim Boragaon, Guwahati, 781035, Assam, India.
Chemical Biology Laboratory I, Life Science Division, Institute of Advanced Study in Science and Technology, Paschim Boragaon, Guwahati, 781035, Assam, India.
Microb Pathog. 2024 Oct;195:106855. doi: 10.1016/j.micpath.2024.106855. Epub 2024 Aug 14.
The present study evaluates the in-vitro antibiofilm activity against the biofilm formed by Staphylococcus aureus, and the wound-healing efficacy of two different types of rhamnolipids produced by Pseudomonas aeruginosa strain JS29 in S.aureus infected wounds. The biosurfactant production was carried out in a mineral salt medium supplemented with 2 % Glucose and 2 % Glycerol individually and thus were designated as RL-Glu and RL-Gly respectively. 0.5 mg/ml of RL-Glu and RL-Gly demonstrated 90 % growth inhibition of S. aureus while exhibiting bactericidal activity at 4 mg/ml of RL-Glu and 1 mg/ml of RL-Gly. Both types of rhamnolipid cause changes in membrane permeability leading to pathogens' non-viability. 90 % inhibition of biofilm formation by S. aureus was observed at 2 mg/ml of RL-Glu and 0.5 mg/ml of RL-Gly, while 0.5 mg/ml of both rhamnolipid disrupted 90 % of the preformed biofilm. 0.5 mg/ml of RL-Glu and RL-Gly decreases the production of exopolysaccharides and also causes structural alteration. 0.5 mg/ml of RL-Glu and RL-Gly were found to exhibit effective wound healing efficacy in S. aureus infected wounds within 7 days of treatment. Histopathological studies of wound sites revealed efficient wound management by both the rhamnolipid. LCMS and GCMS characterization of the biosurfactant revealed that JS29 produces different rhamnolipid congeners when grown on different carbon sources, thereby influencing the antimicrobial, antibiofilm, and wound healing efficacy of rhamnolipid.
本研究评估了两种不同类型的铜绿假单胞菌 JS29 产生的鼠李糖脂在金黄色葡萄球菌感染伤口中的体外抗生物膜活性和伤口愈合功效。生物表面活性剂的生产是在补充有 2%葡萄糖和 2%甘油的无机盐培养基中进行的，分别将其命名为 RL-Glu 和 RL-Gly。0.5mg/ml 的 RL-Glu 和 RL-Gly 对金黄色葡萄球菌的生长抑制率达到 90%，而 RL-Glu 达到 4mg/ml 和 RL-Gly 达到 1mg/ml 时表现出杀菌活性。两种类型的鼠李糖脂都会导致膜通透性发生变化，从而使病原体失去活力。在 2mg/ml 的 RL-Glu 和 0.5mg/ml 的 RL-Gly 作用下，金黄色葡萄球菌生物膜形成的抑制率达到 90%，而 0.5mg/ml 的两种鼠李糖脂则破坏了 90%的已形成生物膜。0.5mg/ml 的 RL-Glu 和 RL-Gly 减少了胞外多糖的产生，同时也导致结构发生改变。研究发现，0.5mg/ml 的 RL-Glu 和 RL-Gly 在金黄色葡萄球菌感染的伤口中具有有效的伤口愈合功效，在治疗 7 天内即可见效。对伤口部位的组织病理学研究表明，两种鼠李糖脂都能有效地进行伤口管理。通过 LCMS 和 GCMS 对生物表面活性剂进行的表征揭示，JS29 利用不同的碳源生长时会产生不同的鼠李糖脂同系物，从而影响鼠李糖脂的抗菌、抗生物膜和伤口愈合功效。
