Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE, Brazil.
School of Pharmacy, Laboratory of Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil.
Future Microbiol. 2024;19(8):667-679. doi: 10.2217/fmb-2023-0271. Epub 2024 Jun 12.
The present study investigated the antimicrobial effectiveness of a rhamnolipid complexed with arginine (RLMIX_Arg) against planktonic cells and biofilms of methicillin-resistant (MRSA). Susceptibility testing was performed using the Clinical & Laboratory Standards Institute protocol: M07-A10, checkerboard test, biofilm in plates and catheters and flow cytometry were used. RLMIX_Arg has bactericidal and synergistic activity with oxacillin. RLMIX_Arg inhibits the formation of MRSA biofilms on plates at sub-inhibitory concentrations and has antibiofilm action against MRSA in peripheral venous catheters. Catheters impregnated with RLMIX_Arg reduce the formation of MRSA biofilms. RLMIX_Arg exhibits potential for application in preventing infections related to methicillin-resistant biofilms.
本研究调查了精氨酸复合鼠李糖脂（RLMIX_Arg）对耐甲氧西林金黄色葡萄球菌（MRSA）浮游细胞和生物膜的抗菌效果。采用临床和实验室标准协会（CLSI）协议 M07-A10 进行药敏试验：棋盘试验、平板和导管生物膜以及流式细胞术。RLMIX_Arg 对 oxacillin 具有杀菌和协同作用。RLMIX_Arg 在亚抑菌浓度下抑制 MRSA 生物膜在平板上的形成，对外周静脉导管中的 MRSA 具有抗生物膜作用。用 RLMIX_Arg 浸渍的导管可减少 MRSA 生物膜的形成。RLMIX_Arg 具有在预防与耐甲氧西林生物膜相关的感染方面的应用潜力。
