School of Food Science & Chemical Engineering, Zhengzhou University of Technology, Zhengzhou, He'nan 450044, China.
School of Food Science and Technology, Henan University of Technology, Zhengzhou, He'nan 450001, China.
Int J Biol Macromol. 2024 Oct;278(Pt 2):134757. doi: 10.1016/j.ijbiomac.2024.134757. Epub 2024 Aug 14.
Saussurea laniceps is a traditional medicinal herb. In our previous study, a pectin polysaccharide, SLP-4, was isolated from the petals of S. laniceps. In this study, the immunomodulatory activity of SLP-4 was studied by analyzing its effects on macrophage (RAW 264.7 cells) polarization. The immunomodulatory activity assays indicated that SLP-4 could significantly enhance the pinocytic and phagocytic capacity and promote the expression and secretion of cytotoxic molecules (nitric oxide, increased by 6.4 times when the SLP-4 concentration was 800 μg/mL) and cytokines (tumor necrosis factor-α and interleukin-6 increased by 7.7 and 11.9 times, respectively) in original macrophage. The possible mechanism could be attributed to the activation of the mitogen-activated protein kinase and nuclear factor-κB signaling pathways through Toll-like receptors 2 and 4. Moreover, SLP-4 significantly induced M1 polarization of original macrophages and transferred macrophages from M2 to M1, but had little effect on the conversion of M1 macrophages into M2 phenotype. Overall, these results demonstrate the potential of SLP-4 as an attractive immunomodulating functional supplement.
雪莲是一种传统的药用植物。在我们之前的研究中，从雪莲的花瓣中分离出一种果胶多糖 SLP-4。在这项研究中，通过分析其对巨噬细胞（RAW 264.7 细胞）极化的影响来研究 SLP-4 的免疫调节活性。免疫调节活性测定表明，SLP-4 能显著增强原始巨噬细胞的吞噬能力，并促进细胞毒性分子（当 SLP-4 浓度为 800μg/mL 时，一氧化氮增加 6.4 倍）和细胞因子（肿瘤坏死因子-α和白细胞介素-6 分别增加 7.7 倍和 11.9 倍）的表达和分泌。其可能的机制可归因于 Toll 样受体 2 和 4 通过丝裂原活化蛋白激酶和核因子-κB 信号通路的激活。此外，SLP-4 能显著诱导原始巨噬细胞向 M1 极化，并将巨噬细胞从 M2 向 M1 转化，但对 M1 巨噬细胞向 M2 表型的转化影响不大。总的来说，这些结果表明 SLP-4 作为一种有吸引力的免疫调节功能补充剂具有潜在的应用价值。
