玉竹多糖的结构表征及其对巨噬细胞的免疫调节作用。
Structural Characterization of Polygonatum Cyrtonema Polysaccharide and Its Immunomodulatory Effects on Macrophages.
机构信息
TCM and Ethnomedicine Innovation & Development International Laboratory, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China.
出版信息
Molecules. 2024 Apr 30;29(9):2076. doi: 10.3390/molecules29092076.
A neutral polysaccharide (NPCP) was isolated and purified from by various chromatographic techniques, including DEAE-52 and Sephadex-G100 chromatography. The structure of NPCP was characterized by HPLC, HPGPC, GC-MS, FT-IR, NMR, and SEM. Results showed that NPCP is composed of glucose (55.4%) and galactose (44.6%) with a molecular weight of 3.2 kDa, and the sugar chain of NPCP was →1)-α-D-Glc-(4→1)-β-D-Gal-(3→. In vitro bioactivity experiments demonstrated that NPCP significantly enhanced macrophages proliferation and phagocytosis while inhibiting the M1 polarization induced by LPS as well as the M2 polarization induced by IL-4 and IL-13 in macrophages. Additionally, NPCP suppressed the secretion of IL-6 and TNF-α in both M1 and M2 cells but promoted the secretion of IL-10. These results suggest that NPCP could serve as an immunomodulatory agent with potential applications in anti-inflammatory therapy.
一种中性多糖（NPCP）从 中分离和纯化出来，采用了各种色谱技术，包括 DEAE-52 和 Sephadex-G100 层析。NPCP 的结构通过 HPLC、HPGPC、GC-MS、FT-IR、NMR 和 SEM 进行了表征。结果表明，NPCP 由葡萄糖（55.4%）和半乳糖（44.6%）组成，分子量为 3.2 kDa，NPCP 的糖链为 →1）-α-D-Glc-（4→1）-β-D-Gal-（3→。体外生物活性实验表明，NPCP 显著增强了巨噬细胞的增殖和吞噬作用，同时抑制了 LPS 诱导的 M1 极化以及 IL-4 和 IL-13 诱导的 M2 极化。此外，NPCP 抑制了 M1 和 M2 细胞中 IL-6 和 TNF-α 的分泌，但促进了 IL-10 的分泌。这些结果表明，NPCP 可以作为一种免疫调节剂，在抗炎治疗中有潜在的应用。
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