文献检索文档翻译深度研究
Suppr Zotero 插件Zotero 插件
邀请有礼套餐&价格历史记录

新学期，新优惠

限时优惠:9月1日-9月22日

30天高级会员仅需29元

1天体验卡首发特惠仅需5.99元

了解详情
不再提醒
插件&应用
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
高级版
套餐订阅购买积分包
AI 工具
文献检索文档翻译深度研究
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2025

玉竹多糖的结构表征及其对巨噬细胞的免疫调节作用。

Structural Characterization of Polygonatum Cyrtonema Polysaccharide and Its Immunomodulatory Effects on Macrophages.

机构信息

TCM and Ethnomedicine Innovation & Development International Laboratory, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China.

出版信息

Molecules. 2024 Apr 30;29(9):2076. doi: 10.3390/molecules29092076.

DOI:10.3390/molecules29092076
PMID:38731567
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11085417/
Abstract

A neutral polysaccharide (NPCP) was isolated and purified from by various chromatographic techniques, including DEAE-52 and Sephadex-G100 chromatography. The structure of NPCP was characterized by HPLC, HPGPC, GC-MS, FT-IR, NMR, and SEM. Results showed that NPCP is composed of glucose (55.4%) and galactose (44.6%) with a molecular weight of 3.2 kDa, and the sugar chain of NPCP was →1)-α-D-Glc-(4→1)-β-D-Gal-(3→. In vitro bioactivity experiments demonstrated that NPCP significantly enhanced macrophages proliferation and phagocytosis while inhibiting the M1 polarization induced by LPS as well as the M2 polarization induced by IL-4 and IL-13 in macrophages. Additionally, NPCP suppressed the secretion of IL-6 and TNF-α in both M1 and M2 cells but promoted the secretion of IL-10. These results suggest that NPCP could serve as an immunomodulatory agent with potential applications in anti-inflammatory therapy.

摘要

一种中性多糖（NPCP）从 中分离和纯化出来，采用了各种色谱技术，包括 DEAE-52 和 Sephadex-G100 层析。NPCP 的结构通过 HPLC、HPGPC、GC-MS、FT-IR、NMR 和 SEM 进行了表征。结果表明，NPCP 由葡萄糖（55.4%）和半乳糖（44.6%）组成，分子量为 3.2 kDa，NPCP 的糖链为 →1）-α-D-Glc-（4→1）-β-D-Gal-（3→。体外生物活性实验表明，NPCP 显著增强了巨噬细胞的增殖和吞噬作用，同时抑制了 LPS 诱导的 M1 极化以及 IL-4 和 IL-13 诱导的 M2 极化。此外，NPCP 抑制了 M1 和 M2 细胞中 IL-6 和 TNF-α 的分泌，但促进了 IL-10 的分泌。这些结果表明，NPCP 可以作为一种免疫调节剂，在抗炎治疗中有潜在的应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c1a/11085417/247b7006d747/molecules-29-02076-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c1a/11085417/6d0551d42de7/molecules-29-02076-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c1a/11085417/8ff4c945a479/molecules-29-02076-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c1a/11085417/a17cd7e25221/molecules-29-02076-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c1a/11085417/52777d3b07d7/molecules-29-02076-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c1a/11085417/8d53336ef473/molecules-29-02076-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c1a/11085417/1be2af1404ea/molecules-29-02076-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c1a/11085417/2d4f56a1cd98/molecules-29-02076-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c1a/11085417/247b7006d747/molecules-29-02076-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c1a/11085417/6d0551d42de7/molecules-29-02076-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c1a/11085417/8ff4c945a479/molecules-29-02076-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c1a/11085417/a17cd7e25221/molecules-29-02076-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c1a/11085417/52777d3b07d7/molecules-29-02076-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c1a/11085417/8d53336ef473/molecules-29-02076-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c1a/11085417/1be2af1404ea/molecules-29-02076-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c1a/11085417/2d4f56a1cd98/molecules-29-02076-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c1a/11085417/247b7006d747/molecules-29-02076-g009.jpg

相似文献

[1]
Structural Characterization of Polygonatum Cyrtonema Polysaccharide and Its Immunomodulatory Effects on Macrophages.

Molecules. 2024-4-30

[2]
Purification, characterization and immunomodulatory activity of fructans from Polygonatum odoratum and P. cyrtonema.

Carbohydr Polym. 2019-3-8

[3]
Effects of different steaming times on the composition, structure and immune activity of Polygonatum Polysaccharide.

J Ethnopharmacol. 2023-6-28

[4]
Chemical structures and immunomodulatory activities of polysaccharides from Polygonatum kingianum.

Int J Biol Macromol. 2024-11

[5]
Purification, characterization and immunomodulatory activity of a polysaccharide from Celosia cristata.

Carbohydr Polym. 2015-7-8

[6]
Structural characterization and immunomodulating activities of a novel polysaccharide from Nervilia fordii.

Int J Biol Macromol. 2018-3-23

[7]
Structural characterization and innate immunomodulatory effect of glucomannan from Bletilla striata.

Int J Biol Macromol. 2024-7

[8]
Structure and antitumor and immunomodulatory activities of a water-soluble polysaccharide from Dimocarpus longan pulp.

Int J Mol Sci. 2014-3-24

[9]
Water-soluble polysaccharide from Erythronium sibiricum bulb: Structural characterisation and immunomodulating activity.

Int J Biol Macromol. 2017-7-12

[10]
Structure characteristics and immunomodulatory activities of a polysaccharide RGRP-1b from radix ginseng Rubra.

Int J Biol Macromol. 2021-10-31

引用本文的文献

[1]
Health benefits and application of polysaccharides from Hua.

Front Pharmacol. 2025-7-18

[2]
Preparation, Structure, Function, and Application of Dietary Polysaccharides from in the Food Industry: A Review.

Molecules. 2025-2-27

本文引用的文献

[1]
TNF-α versus IL-6 Genes Expression levels in Active Rheumatoid Arthritis: Clinical and Laboratory Determinants.

Egypt J Immunol. 2023-10

[2]
Structural characterization and anti-inflammatory activity of a novel polysaccharide PKP2-1 from .

Front Nutr. 2023-3-27

[3]
Structural characterization and induction of tumor cell apoptosis of polysaccharide from purple sweet potato (Ipomoea batatas (L.) Lam).

Int J Biol Macromol. 2023-4-30

[4]
Structure characterization and immunomodulatory activity of a polysaccharide from Saposhnikoviae Radix.

Int J Biol Macromol. 2023-4-1

[5]
Anti-inflammatory effects and molecular mechanisms of bioactive small molecule garlic polysaccharide.

Front Nutr. 2023-1-9

[6]
Polysaccharides of Plantago asiatica enhance antitumor activity via regulating macrophages to M1-like phenotype.

Biomed Pharmacother. 2023-3

[7]
M2 tumor-associated macrophage mediates the maintenance of stemness to promote cisplatin resistance by secreting TGF-β1 in esophageal squamous cell carcinoma.

J Transl Med. 2023-1-14

[8]
Antitumor effect of a polysaccharide from Pseudostellaria heterophylla through reversing tumor-associated macrophages phenotype.

Int J Biol Macromol. 2022-11-1

[9]
A Review of Mill. Genus: Its Taxonomy, Chemical Constituents, and Pharmacological Effect Due to Processing Changes.

Molecules. 2022-7-28

[10]
Anti-cancer Potential of Polysaccharide Extracted From on HepG2 Cells via Cell Cycle Arrest and Apoptosis.

Front Nutr. 2022-7-4

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

推荐工具

医学文档翻译智能文献检索