Laboratorio de Biología Molecular, Instituto de Investigaciones en Ciencias Agrarias de Rosario (IICAR) CONICET-UNR, Facultad de Ciencias Agrarias, Campo Experimental Villarino, Universidad Nacional de Rosario, Zavalla (S2125ZAA), Santa Fe, Argentina.
DIADE, Univ. Montpellier, CIRAD, IRD, Montpellier, France.
Sci Data. 2024 Aug 16;11(1):891. doi: 10.1038/s41597-024-03731-0.
Paspalum notatum Flüggé is an economically important subtropical fodder grass that is widely used in the Americas. Here, we report a new chromosome-scale genome assembly and annotation of a diploid biotype collected in the center of origin of the species. Using Oxford Nanopore long reads, we generated a 557.81 Mb genome assembly (N50 = 56.1 Mb) with high gene completeness (BUSCO = 98.73%). Genome annotation identified 320 Mb (57.86%) of repetitive elements and 45,074 gene models, of which 36,079 have a high level of confidence. Further characterisation included the identification of 59 miRNA precursors together with their putative targets. The present work provides a comprehensive genomic resource for P. notatum improvement and a reference frame for functional and evolutionary research within the genus.
雀稗是一种重要的亚热带饲料草，在美洲被广泛应用。本文报道了一种在雀稗种源中心收集的二倍体生物型的全新染色体水平的基因组组装和注释。利用 Oxford Nanopore 长读长技术，我们生成了一个 557.81 Mb 的基因组组装（N50 = 56.1 Mb），其基因完整性高（BUSCO = 98.73%）。基因组注释鉴定了 320 Mb（57.86%）的重复序列和 45074 个基因模型，其中 36079 个具有高度可信度。进一步的特征分析包括鉴定了 59 个 miRNA 前体及其潜在靶标。本研究为雀稗的改良提供了全面的基因组资源，并为该属的功能和进化研究提供了参考框架。
