College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China.
Suzhou Institute for Advanced Research, University of Science and Technology of China, Suzhou 215123, China.
ACS Appl Mater Interfaces. 2024 Aug 28;16(34):44341-44349. doi: 10.1021/acsami.4c10625. Epub 2024 Aug 17.
Oxidase enzyme-based electrochemical bioassays have garnered considerable interest due to their specificity and high efficiency. However, in traditional solid-liquid diphase enzyme electrode systems, the low solubility of oxygen and its slow mass transfer rate limit the oxidase catalytic reaction kinetics, thereby affecting the bioassay performance, including the detection accuracy, sensitivity, and linear dynamic range. ZIF-8 nanoparticles (NPs) possess hydrophobic and high-porosity characteristics, enabling them to serve as oxygen nanocarriers. In this work, we constructed a solid-liquid-air triphase enzyme electrode by encapsulating ZIF-8 NPs within an oxidase network. Hydrophobic ZIF-8 NPs can provide a rapid and sufficient supply of oxygen for the oxidase-catalyzed reactions, which enhances and stabilizes the kinetics of oxidase-catalyzed reactions. This approach eliminates the issue of "oxygen deficiency" at the traditional solid-liquid diphase interface. Consequently, the triphase enzyme electrode exhibits a 12-fold higher linear detection range than the diphase system and possesses good detection accuracy in electrolytes even with fluctuating oxygen levels. This work proposes a novel approach to construct triphase reaction systems for addressing the gas deficiency problem in heterogeneous catalysis.
基于氧化酶的电化学生物分析因其特异性和高效率而受到广泛关注。然而，在传统的固-液二相酶电极系统中，氧气的低溶解度和缓慢的传质速率限制了氧化酶的催化反应动力学，从而影响了生物分析性能，包括检测精度、灵敏度和线性动态范围。ZIF-8 纳米粒子（NPs）具有疏水性和高多孔性的特点，可用作氧气纳米载体。在这项工作中，我们通过将 ZIF-8 NPs 封装在氧化酶网络中构建了固-液-气三相酶电极。疏水性 ZIF-8 NPs 可为氧化酶催化反应提供快速且充足的氧气供应，从而增强和稳定氧化酶催化反应的动力学。这种方法消除了传统固-液二相界面处的“缺氧”问题。因此，三相酶电极的线性检测范围比二相系统高 12 倍，并且即使在氧气水平波动的情况下，在电解质中也具有良好的检测精度。这项工作提出了一种构建三相反应系统的新方法，用于解决多相催化中的气体短缺问题。
