College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China.
Nanoscale Horiz. 2019 Jan 1;4(1):231-235. doi: 10.1039/c8nh00184g. Epub 2018 Oct 5.
Gaseous reactants play a key role in a wide range of biocatalytic reactions, however reaction kinetics are generally limited by the slow mass transport of gases (typically oxygen) in or through aqueous solutions. Inspired by the morphologies of natural non-wetting surfaces, herein we address this limitation by developing a triphase reaction system possessing a triphase gas-solid-liquid interface. As a proof of concept, we study the kinetics of glucose oxidase (GOx) catalyzed reactions using a triphase system fabricated by layering GOx upon superhydrophobic mesoporous ZnO nanowire arrays through which oxygen, needed for the enzymatic reaction, is supplied directly from the atmosphere to the liquid-solid interface. We find that the enzymatic reaction rate is enhanced by a factor of 30 over that obtained from a conventional diphase system where oxygen is supplied through and from the liquid. The triphase system offers the opportunity to develop high performance bioassay systems, serving as an enabling platform for addressing challenges posed by gas-deficit kinetics.
气态反应物在广泛的生物催化反应中起着关键作用，然而反应动力学通常受到气体（通常是氧气）在水溶液中或通过水溶液的缓慢传质的限制。受天然非润湿表面形态的启发，本文通过开发具有三相气-固-液界面的三相反应系统来解决这一限制。作为概念验证，我们通过在超疏水介孔 ZnO 纳米线阵列上分层葡萄糖氧化酶 (GOx) 来研究三相系统中 GOx 催化反应的动力学，其中酶反应所需的氧气直接从大气供应到液-固界面。我们发现，与通过和从液体中供应氧气的传统两相系统相比，酶反应速率提高了 30 倍。三相系统为开发高性能生物分析系统提供了机会，为解决由气体缺乏动力学带来的挑战提供了一个使能平台。
