Center of Excellence in Molecular Crop, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.
Plant Secondary Metabolism, Division of Biological Science, NARA Institute of Science and Technology, Nara, Japan.
Plant Cell Rep. 2024 Aug 17;43(9):217. doi: 10.1007/s00299-024-03302-y.
Approximately 119 MADS-box genes have been identified in durian. Moreover, DzAGL6-1 primarily expressed during fruit development, activates the DzPSY promoter. Transient expression of DzAGL6-1 in tomatoes influences carotenoid production. MADS-box transcription factors play a crucial role in regulating plant biological processes, including fruit ripening and associated events. This study aimed to comprehend the mechanisms involved in durian fruit development and ripening and carotenoid production by conducting a genome-wide analysis of MADS-box proteins in durian (Durio zibethinus L.), an economically important fruit in Southeast Asia. A total of 119 durian MADS-box proteins were identified from the genome of the 'Musang King' cultivar. Based on the phylogenetic analysis, the proteins were classified into types I and II, which exhibited similar conserved motif compositions. Notably, only 16 durian MADS-box genes exhibited fruit-specific expression patterns. Among these genes, DzAGL6-1 was predominantly expressed during fruit development, a stage at which carotenoid biosynthesis is activated. Transient expression of DzAGL6-1 in tomato fruit increased the transcript level of the carotenoid biosynthetic gene phytoene synthase (PSY) and the β-carotene content. Furthermore, DzAGL6-1 activated the promoter activity of DzPSY, as demonstrated by a dual-luciferase assay. These findings provide insights into the role of MADS-box transcription factors in regulating carotenoid biosynthesis during durian fruit development.
约 119 个 MADS-box 基因已在榴梿中被鉴定。此外，DzAGL6-1 主要在果实发育过程中表达，激活 DzPSY 启动子。DzAGL6-1 在番茄中的瞬时表达影响类胡萝卜素的产生。MADS-box 转录因子在调控植物生物过程中发挥着重要作用，包括果实成熟和相关事件。本研究旨在通过对东南亚重要经济水果榴梿（Durio zibethinus L.）的 MADS-box 蛋白进行全基因组分析，来理解榴梿果实发育、成熟和类胡萝卜素产生的机制。从“猫山王”品种的基因组中鉴定出 119 种榴梿 MADS-box 蛋白。基于系统发生分析，这些蛋白被分为 I 型和 II 型，它们表现出相似的保守基序组成。值得注意的是，只有 16 个榴梿 MADS-box 基因表现出果实特异性表达模式。在这些基因中，DzAGL6-1 在果实发育过程中表达量最高，此时类胡萝卜素生物合成被激活。DzAGL6-1 在番茄果实中的瞬时表达增加了类胡萝卜素生物合成基因 phytoene synthase（PSY）的转录水平和β-胡萝卜素含量。此外，DzAGL6-1 通过双荧光素酶报告基因实验激活了 DzPSY 启动子的活性。这些发现为 MADS-box 转录因子在调控榴梿果实发育过程中类胡萝卜素生物合成的作用提供了新的见解。
