College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China.
School of Public Health, Lanzhou University, Lanzhou 730000, China.
Food Chem. 2024 Dec 15;461:140863. doi: 10.1016/j.foodchem.2024.140863. Epub 2024 Aug 13.
A critical transcription factor, PacC, modulates the expression of fungal pH signaling. Although PacC-mediated environmental pH has been reported to regulate the growth and pathogenicity of postharvest pathogens, the involvement of PacC in sucrose metabolism, sugar transport, and the pentose phosphate pathway (PPP) in different zones of decayed fruit remains unclear. Our work showed that the inoculation with a PePacC deletion strain of Penicillium expansum (ΔPePacC) accelerated sucrose catabolism and glucose and fructose accumulation in different zones of apple fruit. This was attributed to an increase in sucrose metabolism enzyme activities and up-regulation of the sugar transporter protein-related gene expression. Moreover, ΔPePacC inoculation increased the PPP-related enzyme activities and the levels of nicotinamide adenine dinucleotide phosphate (NADPH) and NADP. In conclusion, PacC modulates sucrose metabolism, sugar transport, and the PPP in apple fruit by mediating dynamic changes in environmental pH, thereby enhancing fruit disease resistance.
一个关键的转录因子，PacC，调节真菌 pH 信号的表达。虽然已经报道 PacC 介导的环境 pH 调节采后病原菌的生长和致病性，但 PacC 在腐烂果实不同区域的蔗糖代谢、糖转运和戊糖磷酸途径（PPP）中的参与仍不清楚。我们的工作表明，接种扩展青霉的 PePacC 缺失菌株（ΔPePacC）加速了不同区域苹果果实中蔗糖的分解代谢以及葡萄糖和果糖的积累。这归因于蔗糖代谢酶活性的增加和糖转运蛋白相关基因表达的上调。此外，ΔPePacC 接种增加了 PPP 相关酶活性以及烟酰胺腺嘌呤二核苷酸磷酸（NADPH）和 NADP 的水平。总之，PacC 通过调节环境 pH 的动态变化来调节苹果果实中的蔗糖代谢、糖转运和 PPP，从而增强果实的抗病性。
