Laboratory of Molecular Immunology and Infectious Disease, Joint Graduate School of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi, 753-8515, Japan; Research Institute for Cell Design Medical Science, Yamaguchi University, 1677-1 Yoshida, Yamaguchi, 753-8515, Japan.
Laboratory of Molecular Immunology and Infectious Disease, Joint Graduate School of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi, 753-8515, Japan.
Biochem Biophys Res Commun. 2024 Dec 17;738:150521. doi: 10.1016/j.bbrc.2024.150521. Epub 2024 Aug 9.
Endogenous retroviruses (ERVs) are remnants of ancient retroviral infections in vertebrate genomes and are inherited by offspring. ERVs can produce pathogenic viruses through gene mutations or recombination. ERVs in domestic cats (ERV-DCs) generate feline leukemia virus subgroup D (FeLV-D) through viral recombination. Herein, we characterized the locus ERV-DC8, on chromosome B1, as an infectious replication-competent provirus. ERV-DC8 infected several cell lines, including human cells. Transmission electron microscopy of ERV-DC8 identified the viral release as a Gammaretrovirus. ERV-DC8 was identified as the FeLV-D viral interference group, with feline copper transporter 1 as its viral receptor. Insertional polymorphism analysis showed high ERV-DC8 integration in domestic cats. This study highlights the role, pathogenicity, and evolutionary relationships between ERVs and their hosts.
内源性逆转录病毒（ERVs）是脊椎动物基因组中古老逆转录病毒感染的残余物，通过后代遗传。ERVs 可以通过基因突变或重组产生致病病毒。家猫中的 ERV（ERV-DC）通过病毒重组产生了猫白血病病毒亚群 D（FeLV-D）。在此，我们将染色体 B1 上的 ERV-DC8 基因座鉴定为具有感染性复制能力的前病毒。ERV-DC8 感染了多种细胞系，包括人类细胞。ERV-DC8 的透射电子显微镜观察显示，病毒释放是一种 gammaretrovirus。ERV-DC8 被鉴定为 FeLV-D 病毒干扰群，其病毒受体是猫铜转运蛋白 1。插入多态性分析表明，ERV-DC8 在家猫中的整合率很高。本研究强调了 ERV 与其宿主之间的作用、致病性和进化关系。
