Lakshmi Narasimhan Aditya, Lee Nakyeong, Kim Sangui, Kim Young-Eun, Christabel Catherine, Yu Hyoji, Kim Eui-Jin, Oh You-Kwan
School of Chemical Engineering, Pusan National University (PNU), Busan 46241, Republic of Korea.
Institute for Environment & Energy, PNU, Busan 46241, Republic of Korea; Division of Environmental Materials, Honam National Institute of Biological Resources (HNIBR), Mokpo 58762, Republic of Korea.
Bioresour Technol. 2024 Nov;411:131301. doi: 10.1016/j.biortech.2024.131301. Epub 2024 Aug 15.
This study investigated the technical feasibility of using electrogermination to activate dormant cysts as an inoculum for subsequent 14-d photosynthetic astaxanthin production in Haematococcus lacustris. Electrotreatment affected the cell viability, surface charge, and morphology of H. lacustris cysts. At an optimal voltage of 2 V for 60 min, the cyst germination rate peaked at 44.6 % after 1 d, representing a 2.2-fold increase compared with that of the untreated control. Notably, electrogermination significantly enhanced both the astaxanthin content (44.9 mg/g cell) and productivity (13.2 mg/L/d) after 14 d of photobioreactor cultivation, corresponding to 1.7- and 1.5-fold increases compared with those in control, respectively. However, excessive electrotreatment, particularly at voltages exceeding 2 V or for durations beyond 60 min, did not enhance the astaxanthin production capability of H. lacustris. Proper optimization of renewable electrogermination can enable sustainable algal biorefinery to produce multiple bioactive products without compromising cell viability and astaxanthin productivity.
本研究调查了利用电萌发激活休眠囊肿作为接种物，用于后续在湖生红球藻中进行14天光合虾青素生产的技术可行性。电处理影响了湖生红球藻囊肿的细胞活力、表面电荷和形态。在2 V的最佳电压下处理60分钟，1天后囊肿萌发率达到峰值44.6%，与未处理的对照组相比提高了2.2倍。值得注意的是，在光生物反应器培养14天后，电萌发显著提高了虾青素含量（44.9 mg/g细胞）和生产率（13.2 mg/L/d），分别比对照组提高了1.7倍和1.5倍。然而，过度的电处理，特别是电压超过2 V或处理时间超过60分钟，并没有提高湖生红球藻的虾青素生产能力。对可再生电萌发进行适当优化，可以实现可持续的藻类生物精炼，生产多种生物活性产品，而不影响细胞活力和虾青素生产率。
