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附着培养法有效诱导雨生红球藻积累虾青素。

The effective photoinduction of Haematococcus pluvialis for accumulating astaxanthin with attached cultivation.

机构信息

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China.

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China.

出版信息

Bioresour Technol. 2014 Jul;163:26-32. doi: 10.1016/j.biortech.2014.04.017. Epub 2014 Apr 16.

Abstract

As the optimal source of astaxanthin, Haematococcus pluvialis was cultured for commercial production of astaxanthin through two continuous phases: cell growth and astaxanthin induction. In this study, the efficiency of an attached system for producing astaxanthin from H. pluvialis was investigated and compared to that of the suspended system (bubble column bioreactor) under various conditions. Results showed that this attached system is more suitable for photoinduction of H. pluvialis than the suspended bioreactor. Under the optimal conditions, the astaxanthin productivity of the attached system was 65.8 mg m(-2)d(-1) and 2.4-fold of that in the suspended system. This attached approach also offers other advantages over suspended systems, such as, producing astaxanthin under a wide range of light intensities and temperatures, saving water, ease to harvest cells, resisting contamination. Therefore, the attached approach can be considered an economical, environmentally friendly and highly-efficient technology for producing astaxanthin from H. pluvialis.

摘要

雨生红球藻是虾青素的最佳来源,通过两个连续的阶段来培养用于商业生产虾青素:细胞生长和虾青素诱导。在这项研究中,研究了附着系统用于生产雨生红球藻虾青素的效率,并将其与悬浮系统(鼓泡柱生物反应器)在各种条件下进行了比较。结果表明,与悬浮生物反应器相比,该附着系统更适合于雨生红球藻的光诱导。在最佳条件下,附着系统的虾青素生产力为 65.8 mg m(-2)d(-1),是悬浮系统的 2.4 倍。与悬浮系统相比,这种附着方法还具有其他优势,例如,在广泛的光照强度和温度范围内生产虾青素、节约用水、易于收获细胞、抗污染。因此,附着方法可以被认为是一种经济、环保和高效的从雨生红球藻生产虾青素的技术。

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