College of Chemistry, Zhengzhou University, Zhengzhou, 450001, China.
Institute of Chemical Sciences, Bahauddin Zakariya University, Multan, 60800, Pakistan.
Sci Rep. 2024 Aug 17;14(1):19068. doi: 10.1038/s41598-024-70052-y.
Herein we have reported a fluorescent probe (MB-M) based on MB derivative for Cu ions detection. The probe was well characterized by H NMR, C NMR and HR-MS spectrum. Probe MB-M showed naked-eyes recognition to Cu as color change from colorless to indigo. The probe exhibited promising features such as high fluorescence and UV-vis selectivity, fast response (5 mint), workable at pH 2-7, and low limit of detection (LOD = 0.33 µM). Probe MB-M was also used for Cu ions imaging in HepG-2 cells and detection in daily life (Test Strip and lake water). Moreover, non-covalent interaction (NCI) and quantum theory of atoms in molecules (QTAIM) analysis were used to study the interaction between MB-M and Cu ions. By examining the electronic characteristics of the complex using natural bond orbital (NBO), electron density difference (EDD), and frontier molecular orbital (FMO) analysis, the sensitivity of MB-M towards Cu ions were investigated. The results illustrated that the interactions between MB-M and Cu ions involved chemisorption.
在此，我们报道了一种基于 MB 衍生物的荧光探针（MB-M）用于 Cu 离子的检测。探针通过 1H NMR、13C NMR 和高分辨质谱进行了很好的表征。探针 MB-M 对 Cu 具有裸眼识别能力，颜色从无色变为靛蓝色。该探针具有高荧光和紫外可见选择性、快速响应（5 分钟）、在 pH 2-7 范围内工作、检测限低（LOD=0.33 μM）等优点。探针 MB-M 还用于 HepG-2 细胞中的 Cu 离子成像和日常生活中的检测（测试条和湖水）。此外，还使用非共价相互作用（NCI）和原子分子量子理论（QTAIM）分析来研究 MB-M 和 Cu 离子之间的相互作用。通过使用自然键轨道（NBO）、电子密度差（EDD）和前沿分子轨道（FMO）分析来检查配合物的电子特性，研究了 MB-M 对 Cu 离子的敏感性。结果表明，MB-M 与 Cu 离子之间的相互作用涉及化学吸附。
