Das Shimul, Jarugula Sridhar, Bagewadi Basavaraj, Fayad Amer, Karasev Alexander V, Naidu Rayapati A
Department of Plant Pathology, Washington State University, Irrigated Agriculture Research and Extension Center, Prosser, WA, U.S.A.
Agrotechnology Discipline, Khulna University, Khulna, Bangladesh.
Plant Dis. 2024 Dec;108(12):3453-3462. doi: 10.1094/PDIS-05-24-1113-SR. Epub 2024 Nov 18.
Bean common mosaic virus (BCMV) is causing economically important diseases in leguminous crops worldwide. In this study, BCMV isolates from country bean (CB; ), yard-long bean (YLB; ), and rajma bean (RB; ) collected from Bangladesh, Nepal, and Cambodia were characterized. Samples that tested positive for BCMV in serological assays were subjected to high-throughput sequencing to generate near-full-length genome sequences. In pairwise comparisons of the polyprotein open reading frame, 13 BCMV isolates from Bangladesh, Cambodia, and Nepal showed sequence identity of 92.1 to 98.8% at the nucleotide and 94.2 to 99% at the amino acid level among themselves and with corresponding sequences of BCMV reported previously. In phylogenetic analyses using the global BCMV sequences, they segregated into five distinct lineages, with RB isolates from Nepal clustering with US1/NL1-clade of common bean isolates from different countries, YLB isolates aligning with blackeye cowpea strain sequences reported from China, and CB isolates from Nepal and Bangladesh clustering with soybean isolates from China. One YLB isolate from Nepal was identified as a putative recombinant. None of the BCMV sequences aligned with isolates representing the RU1 or PStV clades. In grow-out tests, seed samples from local markets showed a 14.3 to 38.1% transmission efficiency rate of BCMV with CB seed lots and 9.5 to 33.3% with YLB seed lots.
菜豆普通花叶病毒（BCMV）在全球豆科作物中引发具有重要经济影响的病害。在本研究中，对从孟加拉国、尼泊尔和柬埔寨采集的菜豆（CB）、长豇豆（YLB）和红芸豆（RB）上的BCMV分离株进行了特征分析。血清学检测呈BCMV阳性的样本进行高通量测序以获得近全长基因组序列。在多聚蛋白开放阅读框的两两比较中，来自孟加拉国、柬埔寨和尼泊尔的13个BCMV分离株在核苷酸水平上相互之间以及与先前报道的BCMV相应序列的序列同一性为92.1%至98.8%，在氨基酸水平上为94.2%至99%。在使用全球BCMV序列进行的系统发育分析中，它们分为五个不同的谱系，来自尼泊尔的RB分离株与来自不同国家的普通菜豆分离株的US1/NL1分支聚类，YLB分离株与中国报道的黑眼豇豆株系序列对齐，来自尼泊尔和孟加拉国的CB分离株与来自中国的大豆分离株聚类。来自尼泊尔的一个YLB分离株被鉴定为推定的重组体。没有BCMV序列与代表RU1或PStV分支的分离株对齐。在种植试验中，当地市场的种子样本显示，BCMV在CB种子批次中的传播效率为14.3%至38.1%，在YLB种子批次中的传播效率为9.5%至33.3%。
