皇竹草多糖的体内吸收、体外模拟消化和粪便发酵特性及其对肠道微生物群的影响。
In vivo absorption, in vitro simulated digestion, and fecal fermentation properties of Imperata cylindrica polysaccharides and their effects on gut microbiota.
机构信息
School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, China; National and Local Joint Engineering Laboratory for Synthesis, Harbin Institute of Technology, Harbin, China; School of Medicine and Health, Harbin Institute of Technology, Harbin, China; Chongqing Research Institute, Harbin Institute of Technology, Chongqing, China.
National and Local Joint Engineering Laboratory for Synthesis, Harbin Institute of Technology, Harbin, China; School of Medicine and Health, Harbin Institute of Technology, Harbin, China; Chongqing Research Institute, Harbin Institute of Technology, Chongqing, China.
出版信息
Food Chem. 2024 Dec 15;461:140773. doi: 10.1016/j.foodchem.2024.140773. Epub 2024 Aug 5.
Recently we have investigated polysaccharide from Imperata cylindrica (ICP) for its physicochemical structure and biological activities. However, the digestion characteristics have yet to be understood. This study investigated the digestion and metabolism characteristics of ICP through in vivo fluorescence tracking, in vitro simulated digestion, fecal fermentation experiments, and microbial sequencing. The results showed that ICP significant distribution in the gastrointestinal tract and kidneys. ICP underwent slight degradation during simulated gastric and intestinal digestion. During fecal fermentation, the utilization degree of ICP and the concentration of short-chain fatty acids (SCFAs) increased. ICP promoted the increase of beneficial microbial abundance. To understand the impact of ICP on the integrity and health of intestinal tissues, molecular docking was employed to preliminarily predict the interaction between ICP and key proteins. The results revealed that ICP could recognize and bind to key proteins through high-affinity targeting binding sites.
最近，我们研究了芦竹多糖（Imperata cylindrica，简称 ICP）的理化结构和生物活性。然而，其消化特性尚不清楚。本研究通过体内荧光示踪、体外模拟消化、粪便发酵实验和微生物测序，探究了 ICP 的消化代谢特性。结果表明，ICP 在胃肠道和肾脏中有明显分布。ICP 在模拟胃和肠道消化过程中仅发生轻微降解。在粪便发酵过程中，ICP 的利用率和短链脂肪酸（short-chain fatty acids，简称 SCFAs）的浓度增加。ICP 促进有益微生物丰度的增加。为了了解 ICP 对肠道组织完整性和健康的影响，我们采用分子对接的方法初步预测了 ICP 与关键蛋白的相互作用。结果表明，ICP 可以通过高亲和力靶向结合位点识别和结合关键蛋白。
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