School of Marine Science and Engineering, Nanjing Normal University, Nanjing, Jiangsu Province, 210023, China; School of Ecology and Environment, Anhui Normal University, Wuhu, Anhui Province, 241002, China.
School of Marine Science and Engineering, Nanjing Normal University, Nanjing, Jiangsu Province, 210023, China.
Mar Environ Res. 2024 Oct;201:106689. doi: 10.1016/j.marenvres.2024.106689. Epub 2024 Aug 14.
To assess the toxicity of Hexabromocyclododecane (HBCD), the population, individual, and cellular biochemical parameters of the rotifer Brachionus plicatilis exposed to different concentrations of HBCD were investigated. The results showed that the population growth rate, reproductive period, and offspring number in B. plicatilis significantly decreased under 324 μg/L and 648 μg/L HBCD. Antioxidant enzyme activity and mRNA expression of CAT and Mn-SOD were promoted at low concentrations (32 μg/L and 64 μg/L) and inhibited at high concentrations (324 μg/L and 648 μg/L), while MDA content accumulated continuously with increasing HBCD concentrations, indicating that HBCD induced oxidation imbalance in rotifers. Further evidence was provided by the correlation between DNA fragmentation and physiological changes. The increased intercellular concentration of Ca and the expression of CaM mRNA suggested that HBCD activated pathways related to calcium signaling. In summary, the excessive production of ROS induced by HBCD was considered to be the main cause of reproductive toxicity.
为评估六溴环十二烷（HBCD）的毒性，研究了不同浓度 HBCD 暴露下褶皱臂尾轮虫的种群、个体和细胞生化参数。结果表明，在 324μg/L 和 648μg/L HBCD 下，褶皱臂尾轮虫的种群增长率、生殖期和后代数量显著降低。CAT 和 Mn-SOD 的抗氧化酶活性和 mRNA 表达在低浓度（32μg/L 和 64μg/L）时被促进，而在高浓度（324μg/L 和 648μg/L）时被抑制，而 MDA 含量随着 HBCD 浓度的增加而持续积累，表明 HBCD 导致轮虫氧化失衡。DNA 片段化与生理变化之间的相关性提供了进一步的证据。细胞间 Ca 浓度的增加和 CamRNA 的表达表明，HBCD 激活了与钙信号转导相关的途径。总之，HBCD 引起的 ROS 过量产生被认为是生殖毒性的主要原因。
