IRCCS Neuromed, Pozzilli, Italy.
Dep. of Veterinary Medical Sciences, University of Bologna, Ozzano Emilia, Italy.
Biochim Biophys Acta Bioenerg. 2024 Nov 1;1865(4):149505. doi: 10.1016/j.bbabio.2024.149505. Epub 2024 Aug 16.
Impaired mitochondria cause an impressive decrease in ATP production becoming a common condition of cardiovascular diseases. Myxomatous mitral valve disease (MMVD) is characterized by mitochondrial dysfunction. By a non-invasive procedure of metabolism analysis on peripheral blood mononuclear cells, we exploit ex-vivo studies that directly constitute a translational approach to evaluate the cell bioenergetics. Cell ATP production decreased in the presence of MMVD, whereas glycolysis was unaffected. In MMVD, the mitochondrial activity underwent a significant reduction of basal respiration, maximal respiration, and ATP production. Our results depicted a pathological condition of MMVD characterized by cell metabolism deprived of mitochondrial energy support.
受损的线粒体导致 ATP 生成显著减少，成为心血管疾病的常见情况。黏液样二尖瓣病变（MMVD）的特征是线粒体功能障碍。通过对外周血单个核细胞进行非侵入性代谢分析的程序，我们进行了体外研究，直接构成了评估细胞生物能量学的转化方法。在 MMVD 存在的情况下，细胞 ATP 产生减少，而糖酵解不受影响。在 MMVD 中，线粒体活性经历了基础呼吸、最大呼吸和 ATP 产生的显著减少。我们的结果描绘了 MMVD 的病理状况，其特征是细胞代谢缺乏线粒体能量支持。
