Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China; National Innovation Centre for Synthetic Biology, Tianjin, 300308, China; Department of Chemistry, COMSATS University Islamabad, Park Road, Tarlai Kalan, 45550, Islamabad, Pakistan.
Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China; National Innovation Centre for Synthetic Biology, Tianjin, 300308, China; Department of Applied Bioeconomy, Wrocław University of Environmental and Life Sciences, 50-375, Wrocław, Poland.
Anaerobe. 2024 Oct;89:102900. doi: 10.1016/j.anaerobe.2024.102900. Epub 2024 Aug 21.
This study investigated the codigestion of corn straw (CS) with cow manure (CM), cow digestion solution (CD), and a strain consortium (SC) for enhanced volatile fatty acid (VFA) production. The aims of this study were to develop a sustainable technique to increase VFA yields, examine how combining microbial reagents with CS affects VFA production by functional microorganisms, and assess the feasibility of improving microbial diversity through codigestion.
Batch experiments evaluated VFA production dynamics and microbial community changes with different combinations of CS substrates with CM, CD, and SC. Analytical methods included measuring VFAs by GC, ammonia and chemical oxygen demand (COD) by standard methods and microbial community analysis by 16S rRNA gene sequencing.
Codigesting CS with the strain consortium yielded initial VFA concentrations ranging from 0.6 to 1.0 g/L, which were greater than those of the other combinations (0.05-0.3 g/L). Including CM, and CD further increased VFA production to 1.0-2.0 g/L, with the highest value of 2.0 g/L occurring when all four substrates were codigested. Significant ammonium reduction (194-241 mg/L to 29-37 mg/L) and COD reduction (3310-5250 mg/L to 730-1210 mg/L) were observed. Codigestion with CM and CD had greater Shannon diversity indices (3.19-3.24) than did codigestion with the other consortia (2.26). Bacillota dominated (96.5-99.6 %), with Clostridiales playing key roles in organic matter breakdown.
This study demonstrated the feasibility of improving VFA yields and harnessing microbial diversity through anaerobic codigestion of lignocellulosic and animal waste streams. Codigestion substantially enhanced VFA production, which was dominated by butyrate, reduced ammonium and COD, and enriched fiber-degrading and fermentative bacteria. These findings can help optimize codigestion for sustainable waste management and high-value chemical production.
本研究通过玉米秸秆（CS）与牛粪（CM）、牛消化液（CD）和菌株共混物（SC）的共消化来提高挥发性脂肪酸（VFA）的产量。本研究的目的是开发一种可持续的技术来提高 VFA 的产量，研究将微生物试剂与 CS 结合对功能微生物产生 VFA 的影响，并评估通过共消化来改善微生物多样性的可行性。
批处理实验评估了不同 CS 底物与 CM、CD 和 SC 组合时 VFA 产生动力学和微生物群落变化。分析方法包括用 GC 测量 VFA、用标准方法测量氨和化学需氧量（COD）以及用 16S rRNA 基因测序进行微生物群落分析。
CS 与菌株共混物共消化产生的初始 VFA 浓度范围为 0.6-1.0 g/L，高于其他组合（0.05-0.3 g/L）。包括 CM 和 CD 进一步提高了 VFA 的产量，达到 1.0-2.0 g/L，当所有四种底物共消化时，最高值为 2.0 g/L。观察到显著的氨减少（194-241 mg/L 至 29-37 mg/L）和 COD 减少（3310-5250 mg/L 至 730-1210 mg/L）。与其他共混物相比，CM 和 CD 共消化的 Shannon 多样性指数（3.19-3.24）更高（3.19-3.24）。厚壁菌门占主导地位（96.5-99.6%），梭菌目在有机质分解中起关键作用。
本研究通过木质纤维素和动物废物流的厌氧共消化证明了提高 VFA 产量和利用微生物多样性的可行性。共消化大大提高了 VFA 的产量，主要是丁酸，减少了氨和 COD，并丰富了纤维降解和发酵细菌。这些发现可以帮助优化共消化，以实现可持续的废物管理和高价值化学品的生产。
