使用体外蔬菜模型评估脉冲场消融损伤特征
Evaluation of pulsed field ablation lesion characteristics using an in vitro vegetable model.
作者信息
Steiger Nathaniel A, McClennen Logan, Bilenker Justin, Patino Carlos, Matos Carlos D, Sauer Katherine M, Hoyas Carolina, Tedrow Usha B, Zei Paul C, Romero Jorge E, Sauer William H
机构信息
Cardiac Arrhythmia Service, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.
出版信息
J Interv Card Electrophysiol. 2024 Aug 18. doi: 10.1007/s10840-024-01906-8.
BACKGROUND
In vitro models to evaluate cardiac pulsed field ablation (PFA) have not been well established. We sought to create a standardized vegetable model and staining protocol for assessing unipolar PFA using a surface electrode.
METHODS
We exposed potato slabs to unipolar PFA in a saline bath using a 3.5 mm electrode catheter and grounding pad connected to a custom-built high-voltage generator. Lesions were clearly visualized after staining with 2,3,5-triphenyltetrazolium chloride (TTC) using a timed protocol to reveal a necrotic center and a periphery of electroporated cells with intact mitochondria.
RESULTS
Lesion volume increased linearly with increasing voltage and logarithmically with repetitive PFA applications.
CONCLUSION
The findings observed in this vegetable model using a TTC staining protocol are consistent with findings observed with cardiomyocytes.
背景
用于评估心脏脉冲场消融（PFA）的体外模型尚未得到很好的建立。我们试图创建一种标准化的蔬菜模型和染色方案，以使用表面电极评估单极PFA。
方法
我们使用连接到定制高压发生器的3.5毫米电极导管和接地垫，将土豆切片暴露于盐浴中的单极PFA。使用定时方案用2,3,5-三苯基氯化四氮唑（TTC）染色后，病变清晰可见，以显示坏死中心和线粒体完整的电穿孔细胞外周。
结果
病变体积随电压增加呈线性增加，随重复PFA应用呈对数增加。
结论
使用TTC染色方案在该蔬菜模型中观察到的结果与在心肌细胞中观察到的结果一致。
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