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重组人乳头瘤病毒 16 L1 蛋白与 -叠氮基-苯丙氨酸的工程化。

Engineering of Recombinant Human Papillomavirus 16 L1 Protein for Incorporation with -Azido--Phenylalanine.

机构信息

Department of Biotechnology and Bioengineering, Chonnam National University, Gwangju 61186, Republic of Korea.

Department of Chemical and Biomolecular Engineering, KAIST, Daejeon 34141, Republic of Korea.

出版信息

J Microbiol Biotechnol. 2024 Sep 28;34(9):1926-1932. doi: 10.4014/jmb.2407.07033. Epub 2024 Aug 9.

DOI:10.4014/jmb.2407.07033
PMID:39155395
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11473617/
Abstract

Human papillomavirus (HPV) L1 capsid protein were produced in several host systems, but few studies have focused on enhancing the properties of the L1 protein. In this study, we aimed to produce recombinant Human papillomavirus (HPV) L1 capsid protein containing -azido--phenylalanine (pAzF) in . First, we expressed the maltose-binding protein (MBP)-fused HPV16 L1, and 5 residues in HPV16 L1 protein were selected by the in silico modeling for amber codon substitution. Among the variants of the five locations, we identified a candidate that exhibited significant differences in expression with and without pAzF via genetic code expansion (GCE). The expressed recombinant MBP-HPV16L1 protein was confirmed for incorporation of pAzF and the formation of VLPs was tested in vitro.

摘要

人乳头瘤病毒 (HPV) L1 衣壳蛋白在多种宿主系统中被生产出来，但很少有研究集中在增强 L1 蛋白的特性上。在这项研究中，我们旨在生产含有 -叠氮基苯丙氨酸 (pAzF) 的重组人乳头瘤病毒 (HPV) L1 衣壳蛋白。首先，我们表达了麦芽糖结合蛋白 (MBP) 融合的 HPV16 L1，通过计算机建模选择了 HPV16 L1 蛋白中的 5 个残基进行琥珀终止密码子替换。在这 5 个位置的变体中，我们通过遗传密码扩展 (GCE) 鉴定了一个候选变体，该变体在有无 pAzF 的情况下表达差异显著。表达的重组 MBP-HPV16L1 蛋白被证实掺入了 pAzF，并且在体外测试了 VLPs 的形成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f55/11473617/dfb25738031e/jmb-34-9-1926-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f55/11473617/59e2da010040/jmb-34-9-1926-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f55/11473617/753d72102098/jmb-34-9-1926-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f55/11473617/3eacb31f0753/jmb-34-9-1926-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f55/11473617/dfb25738031e/jmb-34-9-1926-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f55/11473617/59e2da010040/jmb-34-9-1926-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f55/11473617/753d72102098/jmb-34-9-1926-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f55/11473617/3eacb31f0753/jmb-34-9-1926-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f55/11473617/dfb25738031e/jmb-34-9-1926-f4.jpg

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本文引用的文献

1
Development of orthogonal aminoacyl-tRNA synthetase mutant for incorporating a non-canonical amino acid.用于掺入非天然氨基酸的正交氨酰-tRNA合成酶突变体的开发。
AMB Express. 2024 May 24;14(1):60. doi: 10.1186/s13568-024-01706-3.
2
Construction, expression, and in vitro assembly of virus-like particles of L1 protein of human papillomavirus type 52 in Escherichia coli BL21 DE3.人乳头瘤病毒52型L1蛋白病毒样颗粒在大肠杆菌BL21 DE3中的构建、表达及体外组装
J Genet Eng Biotechnol. 2022 Feb 7;20(1):19. doi: 10.1186/s43141-021-00281-5.
3
Highly accurate protein structure prediction with AlphaFold.
利用 AlphaFold 进行高精度蛋白质结构预测。
Nature. 2021 Aug;596(7873):583-589. doi: 10.1038/s41586-021-03819-2. Epub 2021 Jul 15.
4
Genetic Code Expansion: A Brief History and Perspective.遗传密码扩展：简要的历史和展望。
Biochemistry. 2021 Nov 23;60(46):3455-3469. doi: 10.1021/acs.biochem.1c00286. Epub 2021 Jul 1.
5
Multivalent Albumin-Neonatal Fc Receptor Interactions Mediate a Prominent Extension of the Serum Half-Life of a Therapeutic Protein.多价白蛋白-新生儿 Fc 受体相互作用介导治疗性蛋白血清半衰期的显著延长。
Mol Pharm. 2021 Jun 7;18(6):2397-2405. doi: 10.1021/acs.molpharmaceut.1c00231. Epub 2021 May 13.
6
In Vitro Assembly of Virus-Like Particles and Their Applications.病毒样颗粒的体外组装及其应用
Life (Basel). 2021 Apr 10;11(4):334. doi: 10.3390/life11040334.
7
Virus-like particles: preparation, immunogenicity and their roles as nanovaccines and drug nanocarriers.病毒样颗粒：制备、免疫原性及其作为纳米疫苗和药物纳米载体的作用。
J Nanobiotechnology. 2021 Feb 25;19(1):59. doi: 10.1186/s12951-021-00806-7.
8
Current strategies in extending half-lives of therapeutic proteins.目前延长治疗性蛋白半衰期的策略。
J Control Release. 2019 May 10;301:176-189. doi: 10.1016/j.jconrel.2019.02.016. Epub 2019 Mar 5.
9
N-terminal truncations on L1 proteins of human papillomaviruses promote their soluble expression in Escherichia coli and self-assembly in vitro.人乳头瘤病毒 L1 蛋白 N 端截短可促进其在大肠杆菌中的可溶性表达和体外自组装。
Emerg Microbes Infect. 2018 Sep 26;7(1):160. doi: 10.1038/s41426-018-0158-2.
10
Generation of therapeutic protein variants with the human serum albumin binding capacity via site-specific fatty acid conjugation.通过定点脂肪酸结合生成具有人血清白蛋白结合能力的治疗性蛋白变体。
Sci Rep. 2017 Dec 21;7(1):18041. doi: 10.1038/s41598-017-18029-y.