Acay Hilal, Güler Güney İnci, Yildirim Ayfer, Derviş Sibel, Dereli Elif
Mardin Artuklu University, Faculty of Health Science, Department of Nutrition and Dietetics, 47000, Mardin, Turkey.
Mardin Artuklu University, Kızıltepe Vocational High School, Department of Plant and Animal Production, 47000, Mardin, Turkey.
Chem Biodivers. 2024 Dec;21(12):e202401972. doi: 10.1002/cbdv.202401972. Epub 2024 Oct 29.
Growing concerns over the human health and environmental impacts of conventional fungicides, coupled with the escalating challenge of microbial resistance, have fueled the search for sustainable biocontrol strategies against plant pathogens. This study reports, for the first time, the green synthesis and characterization of a novel, eco-friendly nanomaterial, designated Pleurotus eryngii-Lecithin-Chitosan Nanomaterial (PEELCN), derived from P. eryngii extract (PEE), lecithin (L), and chitosan (C). The structural attributes of PEELCN were elucidated using Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA), X-ray Diffraction (XRD), and zeta potential measurements, confirming the successful formation of a stable and uniform nanostructure. The antifungal activity of PEELCN, and PEE, was assessed against five economically important phytopathogenic fungi: Neoscytalidium dimidiatum, Alternaria alternata, Verticillium dahliae, Bipolaris sorokiniana, and Fusarium oxysporum. Both PEE and PEELCN exhibited significant inhibitory effects on the mycelial growth of V. dahliae, B. sorokiniana, and N. dimidiatum, with varying degrees of efficacy. The differential antifungal activity suggests a species-specific mode of action. The findings highlight the promising potential of PEELCN as a sustainable, biocompatible, and cost-effective nanofungicide for the management of plant diseases, with the potential for development into a commercially viable biofungicide for sustainable agriculture.
对传统杀菌剂对人类健康和环境影响的日益关注，再加上微生物耐药性挑战的不断升级，推动了对针对植物病原体的可持续生物防治策略的探索。本研究首次报告了一种新型环保纳米材料的绿色合成与表征，该纳米材料名为杏鲍菇-卵磷脂-壳聚糖纳米材料（PEELCN），由杏鲍菇提取物（PEE）、卵磷脂（L）和壳聚糖（C）制成。使用傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）、热重分析（TGA）、X射线衍射（XRD）和zeta电位测量对PEELCN的结构属性进行了阐明，证实成功形成了稳定且均匀的纳米结构。评估了PEELCN和PEE对五种具有经济重要性的植物致病真菌的抗真菌活性：半知菌亚门真菌、链格孢、大丽轮枝菌、小麦根腐离蠕孢和尖孢镰刀菌。PEE和PEELCN对大丽轮枝菌、小麦根腐离蠕孢和半知菌亚门真菌的菌丝生长均表现出显著的抑制作用，且效果程度不同。这种不同的抗真菌活性表明其作用方式具有物种特异性。研究结果突出了PEELCN作为一种可持续、生物相容性好且具有成本效益的纳米杀菌剂在植物病害管理方面的广阔潜力，有发展成为一种适用于可持续农业的商业可行生物杀菌剂的可能性。
