家蚕核型多角体病毒的多角体蛋白基因。

The polyhedrin gene of Bombyx mori nuclear polyhedrosis virus.

作者信息

Wang H, Xin J H, Gu M Z, Li Z P

出版信息

Sci Sin B. 1985 Oct;28(10):1051-9.

Abstract

Cloned SalI fragments of Bombyx mori nuclear polyhedrosis virus (BmSNPV) DNA were screened with the polyhedrin gene of Autographa californica nuclear polyhedrosis virus as a probe. One positive clone, pBN61, with an insert of 1.65 Kb, was obtained. The HindIII, HpaII and AluI maps of the insert were constructed. Part of its nucleotide sequence has been determined. The 46 amino acid sequence, as determined from the nucleotide sequence, was compared with the reported sequence of BmSNPV polyhedrin. Only one amino acid difference has been found. It is likely that clone pBN61 contains the whole BmSNPV polyhedrin gene.

摘要

以苜蓿银纹夜蛾核型多角体病毒的多角体蛋白基因作为探针，对家蚕核型多角体病毒（BmSNPV）DNA的克隆SalI片段进行筛选。获得了一个阳性克隆pBN61，其插入片段为1.65 Kb。构建了插入片段的HindIII、HpaII和AluI图谱。已测定了其部分核苷酸序列。根据核苷酸序列确定的46个氨基酸序列与已报道的BmSNPV多角体蛋白序列进行了比较。仅发现一个氨基酸差异。克隆pBN61很可能包含完整的BmSNPV多角体蛋白基因。