Mboumba Bouassa Ralph-Sydney, Giorgini Giada, Silvestri Cristoforo, Muller Chanté, Nallabelli Nayudu, Alexandrova Yulia, Durand Madeleine, Tremblay Cécile, El-Far Mohamed, Chartrand-Lefebvre Carl, Messier-Peet Marc, Margolese Shari, Flamand Nicolas, Costiniuk Cecilia T, Di Marzo Vincenzo, Jenabian Mohammad-Ali
Department of Biological Sciences and CERMO-FC Research Centre, Université du Quebec à Montréal, Montreal, QC, Canada.
Infectious Diseases and Immunity in Global Health Program, Research Institute of the McGill University Health Centre, Montreal, QC, Canada.
iScience. 2024 Jul 5;27(8):110456. doi: 10.1016/j.isci.2024.110456. eCollection 2024 Aug 16.
Chronic HIV infection is associated with accelerated coronary artery disease (CAD) due to chronic inflammation. The expanded endocannabinoid system (eCBome) and gut microbiota modulate each other and are key regulators of cardiovascular functions and inflammation. We herein investigated the interplay between plasma eCBome mediators and gut microbiota in people with HIV (PWH) and/or subclinical CAD versus HIV-uninfected individuals. CAD was determined by coronary computed tomography (CT) angiography performed on all participants. Plasma eCBome mediator and fecal microbiota composition were assessed by tandem mass spectrometry and 16S rDNA sequencing, respectively. HIV infection was associated with perturbed plasma eCBome mediators characterized by an inverse relationship between anandamide and -acyl-ethanolamines (NAEs) versus 2-AG and 2-monoacylglycerols (MAGs). Plasma triglyceride levels were positively associated with MAGs. Several fecal bacterial taxa were altered in HIV-CAD+ versus controls and correlated with plasma eCBome mediators. CAD-associated taxonomic alterations in fecal bacterial taxa were not found in PWH.
由于慢性炎症，慢性HIV感染与冠状动脉疾病（CAD）加速有关。扩展的内源性大麻素系统（eCBome）和肠道微生物群相互调节，是心血管功能和炎症的关键调节因子。我们在此研究了HIV感染者（PWH）和/或亚临床CAD患者与未感染HIV个体的血浆eCBome介质与肠道微生物群之间的相互作用。通过对所有参与者进行冠状动脉计算机断层扫描（CT）血管造影来确定CAD。分别通过串联质谱和16S rDNA测序评估血浆eCBome介质和粪便微生物群组成。HIV感染与血浆eCBome介质紊乱有关，其特征是花生四烯乙醇胺和N-酰基乙醇胺（NAEs）与2-花生四烯酸甘油酯（2-AG）和2-单酰甘油（MAGs）之间呈负相关。血浆甘油三酯水平与MAGs呈正相关。与对照组相比，HIV-CAD+患者的几种粪便细菌分类群发生了改变，并与血浆eCBome介质相关。在PWH中未发现粪便细菌分类群中与CAD相关的分类学改变。
