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三种猛禽的特性鉴定及精液冷冻保存

Characterization and seminal cryopreservation of three species of birds of prey.

作者信息

Herrera-Barragán J A, Rodríguez-Montiel I, Rosales-Torres A M, Ávalos-Rodríguez A, Guzmán-Sánchez A, Pérez-Rivero J J

机构信息

Department of Agricultural and Animal Production, Metropolitan Autonomous University-Xochimilco, Calzada del Hueso 1100, col. Villa Quietud, Coyoacán, 04960, Mexico City, Mexico.

MSc in Agricultural Sciences, Metropolitan Autonomous University-Xochimilco, Calzada del Hueso 1100, col. Villa Quietud, Coyoacán, 04960, Mexico City, Mexico.

出版信息

Iran J Vet Res. 2024;25(1):69-73. doi: 10.22099/IJVR.2024.47943.6962.

Abstract

BACKGROUND

Assisted reproduction techniques in birds have contributed to many species' conservation and sustainable use. One of these techniques is semen cryopreservation, which is possible following the discovery of suitable cryoprotectants.

AIMS

This study aimed to characterize the fresh and post-thaw ejaculates of different species of birds of prey.

METHODS

The following species were included in the study: red-tailed hawk () n=3, golden eagle () n=3, and Harris's hawk () n=3. Twenty-five ejaculates were obtained for each species. The percentage of spermatozoa motility, viability, and morphology were evaluated.

RESULTS

Evident differences were observed among the ejaculates of the three species, particularly in sperm length and between the fresh and post-thaw parameters of the same species in which the motility reduced to approximately 40% after thawing. It was demonstrated that sperm cryopreservation of the studied species was possible using the same freezing protocol.

CONCLUSION

This study showed that sperm characteristics could influence the parameters obtained during their conservation, both in the fresh and post-thaw states.

摘要

背景

鸟类辅助生殖技术有助于许多物种的保护和可持续利用。其中一项技术是精液冷冻保存,这在发现合适的冷冻保护剂后成为可能。

目的

本研究旨在表征不同种类猛禽的新鲜精液和冻融后精液。

方法

本研究纳入了以下物种:红尾鹰(n = 3)、金鹰(n = 3)和哈里斯鹰(n = 3)。每个物种获取了25份精液。评估了精子活力、存活率和形态的百分比。

结果

在这三个物种的精液之间观察到明显差异,特别是在精子长度方面,并且在同一物种的新鲜精液和冻融后参数之间也存在差异,其中解冻后活力降低至约40%。结果表明,使用相同的冷冻方案对所研究物种的精子进行冷冻保存是可行的。

结论

本研究表明,精子特征会影响其在新鲜状态和冻融后状态下保存期间获得的参数。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8644/11327643/2ab3f9123a7c/ijvr-25-69-g001.jpg

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