Farinelli Luca, D'Angelo Francesco, Ciccullo Carlo, Manzotti Sandra, Gigante Antonio
Clinical Orthopaedics, Department of Clinical and Molecular Sciences, Università Politecnica delle Marche, Ancona, Italy.
IRCCS INRCA, Ancona, Italy.
Osteoarthr Cartil Open. 2024 Jul 19;6(3):100503. doi: 10.1016/j.ocarto.2024.100503. eCollection 2024 Sep.
Aim of the present study was to compare the presence of Mast Cells (MCs) in synovial samples from gleno-humeral osteoarthritis (OA) and from control group.
Synovial tissue samples were obtained during arthroplasty from 23 patients with gleno-humeral OA due to rotator cuff arthropathy (RCA) and from 20 patients without OA, constituting OA group and control group respectively. Before surgery self-reported pain was assessed using VAS score and OSS was used to value functional ability. Shoulder radiograph (Antero-posterior, Y-view and Grashey views) was evaluated by musculoskeletal radiologist and graded according to modified Samilson-Prieto classification.Synovial tissue, obtained during arthroplasty and arthroscopic procedure, was prepared to immunohistochemical analysis with anti-CD31 and anti-CD117 antibodies, to detect respectively endothelial cells and MCs at 40x magnification. Synovitis scores have been assessed. Under the control of the image processing system the distribution and the total number of vessels and MCs were determined.
The numbers of MCs and the area fraction (20x magnification) occupied by them were significantly higher in OA samples than in control tissue. The synovitis score was higher in OA patients with a positive correlation. Vessels number and area fraction were higher in OA patients than in controls. Analysis of MC number in relation to clinical data indicated positive correlation with the VAS score.
The distribution of MCs on synovium significantly differ between OA and control groups. Despite the design of the study could not conclude the cause-effect relationship, the presence of MCs might have role in OA pathogenesis.
Histological study.
本研究旨在比较盂肱骨关节炎（OA）患者与对照组滑膜样本中肥大细胞（MCs）的存在情况。
在关节置换术中，从23例因肩袖关节病（RCA）导致盂肱骨OA的患者以及20例无OA的患者中获取滑膜组织样本，分别构成OA组和对照组。术前使用视觉模拟评分法（VAS）评估自我报告的疼痛情况，并使用肩关节功能评分（OSS）评估功能能力。由肌肉骨骼放射科医生对肩部X线片（前后位、Y位和Grashey位）进行评估，并根据改良的Samilson-Prieto分类法进行分级。在关节置换术和关节镜检查过程中获取的滑膜组织，用抗CD31和抗CD117抗体进行免疫组织化学分析，以分别在40倍放大倍数下检测内皮细胞和MCs。评估滑膜炎评分。在图像处理系统的控制下，确定血管和MCs的分布及总数。
OA样本中MCs的数量及其所占面积分数（20倍放大倍数下）显著高于对照组织。OA患者的滑膜炎评分更高，且呈正相关。OA患者的血管数量和面积分数高于对照组。MC数量与临床数据的分析表明与VAS评分呈正相关。
OA组和对照组滑膜上MCs的分布存在显著差异。尽管本研究设计无法得出因果关系，但MCs的存在可能在OA发病机制中起作用。
组织学研究。
