Hou Qinghua, Zhong Yanfeng, Liao Mengying, Chen Chao, Li Yanling, Li Xiaoqing, Liu Jixian
Department of Thoracic Surgery, Peking University Shenzhen Hospital, Shenzhen, Guangdong, 518036, China.
Department of Central Laboratory, Peking University Shenzhen Hospital, Shenzhen, Guangdong, 518036, China.
Heliyon. 2024 Jul 24;10(15):e35012. doi: 10.1016/j.heliyon.2024.e35012. eCollection 2024 Aug 15.
, a gene associated with various cancers, is considered a tumor suppressor. However, the role of in lung adenocarcinoma (LUAD) remains unknown. In this study, we aimed to assess the role of in the occurrence and prognosis of LUAD.
Using three-tier HTSeq count RNA sequencing data from The Cancer Genome Atlas, we assessed LIN9 expression for the LUAD dataset using the DESeq2 R package and RT-qPCR experiments. Biological functions were assessed using gene set enrichment analysis (clusterProfiler and GOplot). The expression of and the infiltration of immune cells were assessed by Single-sample gene set enrichment analysis. We conducted correlation study using clinical characteristics and receiver operating characteristic curve analysis. The predictive value of was determined using univariate and multivariate Cox regression as well as Kaplan-Meier analysis. Additionally, functional studies were conducted to validate its role in the progression of LUAD.
Expression of was significantly elevated in LUAD, primarily influencing cell cycle, division, and signaling pathways. High expression correlated positively with the infiltration of Th2 cells and inversely with that of plasmacytoid dendritic cells. Furthermore, was associated with older age and advanced clinical stages, posing risks to overall, progression-free, and disease-specific survival. served as a good diagnostic marker, particularly in females, patients aged over 65, and those with clinical N1-3 and M1 stages. Elevated expression enhanced proliferation, migration, and invasion of LUAD cells.
High expression potentially contributes to LUAD occurrence through cell cycle regulation and chromosomal modification. It promotes the malignant characteristics of LUAD cells and holds prognostic value for affected patients.
，一种与多种癌症相关的基因，被认为是一种肿瘤抑制因子。然而，其在肺腺癌（LUAD）中的作用仍不清楚。在本研究中，我们旨在评估 在LUAD发生和预后中的作用。
使用来自癌症基因组图谱的三层HTSeq计数RNA测序数据，我们使用DESeq2 R包和RT-qPCR实验评估LUAD数据集中的LIN9表达。使用基因集富集分析（clusterProfiler和GOplot）评估生物学功能。通过单样本基因集富集分析评估 的表达和免疫细胞浸润。我们使用临床特征进行相关性研究并绘制受试者工作特征曲线分析。使用单因素和多因素Cox回归以及Kaplan-Meier分析确定 的预测价值。此外，进行功能研究以验证其在LUAD进展中的作用。
在LUAD中的表达显著升高，主要影响细胞周期、分裂和信号通路。高 表达与Th2细胞浸润呈正相关，与浆细胞样树突状细胞浸润呈负相关。此外， 与老年和临床晚期相关，对总生存、无进展生存和疾病特异性生存构成风险。 是一个良好的诊断标志物，特别是在女性、65岁以上患者以及临床N1-3和M1期患者中。 表达升高增强了LUAD细胞的增殖、迁移和侵袭。
高 表达可能通过细胞周期调控和染色体修饰促进LUAD的发生。它促进LUAD细胞的恶性特征，并对受影响患者具有预后价值。
