Kagemann Catherine H, Colocho Gabriela M, Aquadro Charles F
Molecular Biology and Genetics, Cornell University, Ithaca, New York, United States.
MicroPubl Biol. 2024 Aug 2;2024. doi: 10.17912/micropub.biology.001233. eCollection 2024.
( ) is an essential gene that regulates germline stem cell maintenance and germline stem cell daughter cell differentiation in . When is partially functional (hypomorphic), the introduction of rescues the mutant fertility phenotype that would otherwise result in partial sterility. Infection by different variants results in differential rescue of the hypomorph fertility phenotype. We were intrigued by the varying degrees of rescue exhibited in the hypomorph when exposed to different variants, prompting us to investigate whether this phenomenon is attributable to variations in the titers of variants. We found no significant difference in ovarian titer between two variant groups, Mel-like (low hypomorph fertility rescue) and MelCS-like variants (higher hypomorph fertility rescue), at hypomorph peak fertility. However, carcass (whole flies without the ovaries) titer between Mel-like and MelCS-like infected hypomorph differed during peak fertility rescue. A positive correlation emerged between the combined titers of ovarian and carcass infections and fertility, implying a more extensive influence that extends beyond ovarian infection alone.
（ ）是一个关键基因，它在 中调节生殖系干细胞的维持以及生殖系干细胞子细胞的分化。当 部分功能缺失（亚效等位基因）时，引入 可挽救原本会导致部分不育的突变体生育力表型。不同 变体的感染导致 亚效等位基因生育力表型的不同程度挽救。当暴露于不同 变体时， 亚效等位基因表现出不同程度的挽救，这引起了我们的兴趣，促使我们研究这种现象是否归因于 变体滴度的差异。我们发现在 亚效等位基因生育力峰值时，两个 变体组，即 Mel样（低 亚效等位基因生育力挽救）和 MelCS样变体（更高 亚效等位基因生育力挽救）之间的卵巢滴度没有显著差异。然而，在生育力挽救峰值期间， Mel样和 MelCS样感染的 亚效等位基因之间的胴体（不含卵巢的完整果蝇）滴度有所不同。卵巢和胴体感染的联合滴度与生育力之间出现正相关，这意味着其影响范围更广，不仅仅局限于卵巢感染。
