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针对人c-myc原癌基因产物的单克隆抗体的分离

Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.

作者信息

Evan G I, Lewis G K, Ramsay G, Bishop J M

出版信息

Mol Cell Biol. 1985 Dec;5(12):3610-6. doi: 10.1128/mcb.5.12.3610-3616.1985.

DOI:10.1128/mcb.5.12.3610-3616.1985
PMID:3915782
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC369192/
Abstract

Six monoclonal antibodies have been isolated from mice immunized with synthetic peptide immunogens whose sequences are derived from that of the human c-myc gene product. Five of these antibodies precipitate p62c-myc from human cells, and three of these five also recognize the mouse c-myc gene product. None of the antibodies sees the chicken p110gag-myc protein. All six antibodies recognize immunoblotted p62c-myc. These reagents also provide the basis for an immunoblotting assay by which to quantitate p62c-myc in cells.

摘要

已从用合成肽免疫原免疫的小鼠中分离出六种单克隆抗体，这些免疫原的序列源自人c-myc基因产物的序列。其中五种抗体可从人细胞中沉淀出p62c-myc，这五种中的三种还能识别小鼠c-myc基因产物。这些抗体均未识别鸡p110gag-myc蛋白。所有六种抗体均能识别免疫印迹的p62c-myc。这些试剂还为定量细胞中p62c-myc的免疫印迹分析提供了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3590/369192/a18122c5d840/molcellb00142-0292-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3590/369192/a3c5125ac23f/molcellb00142-0291-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3590/369192/6ab0f342cb1a/molcellb00142-0291-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3590/369192/f808c74ea42c/molcellb00142-0292-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3590/369192/a18122c5d840/molcellb00142-0292-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3590/369192/a3c5125ac23f/molcellb00142-0291-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3590/369192/6ab0f342cb1a/molcellb00142-0291-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3590/369192/f808c74ea42c/molcellb00142-0292-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3590/369192/a18122c5d840/molcellb00142-0292-b.jpg

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