Department of Clinical Laboratory, The Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu, China.
mSystems. 2024 Sep 17;9(9):e0058624. doi: 10.1128/msystems.00586-24. Epub 2024 Aug 19.
Nontyphoidal (NTS) is the main etiological agent of human nontyphoidal salmonellosis. The aim of this study was to analyze the epidemiological characteristics and horizontal transfer mechanisms of antimicrobial resistance (AMR) genes from eight strains of NTS detected in Zhenjiang City, Jiangsu Province, China. Fecal samples from outpatients with food-borne diarrhea were collected in 2022. The NTS isolates were identified, and their susceptibility was tested with the Vitek 2 Compact system. The genomes of the NTS isolates were sequenced with the Illumina NovaSeq platform and Oxford Nanopore Technologies platform. The AMR genes and mobile genetic elements (MGEs) were predicted with the relevant open access resources. Eight strains of NTS were isolated from 153 specimens, and Typhimurium ST19 was the most prevalent serotype. The AMR gene with the highest detection rate was AAC(6)-Iaa (10.5%) followed by TEM-1 (7.9%), sul2 (6.6%), and tet(A) (5.3%). Eleven MGEs carrying 34 AMR genes were identified on the chromosomes of 3 of the 8 NTS, including 3 resistance islands, 6 composite transposons (Tns), and 2 integrons. Eighteen plasmids carrying 40 AMR genes were detected in the 8 NTS strains, including 6 mobilizable plasmids, 3 conjugative plasmids, and 9 nontransferable plasmids, 7 of which carried 10 composite Tns and 3 integrons. This study provided a theoretical basis, from a genetic perspective, for the prevention and control of NTS resistance in Zhenjiang City.
Human nontyphoidal salmonellosis is one of the common causes of bacterial food-borne illnesses, with significant social and economic impacts, especially those caused by invasive multidrug-resistant nontyphoidal , which entails high morbidity and mortality. Antimicrobial resistance is mainly mediated by drug resistance genes, and mobile genetic elements play key roles in the capture, accumulation, and dissemination of antimicrobial resistance genes. Therefore, it is necessary to study the epidemiological characteristics and horizontal transfer mechanisms of antimicrobial resistance genes of nontyphoidal to prevent the spread of multidrug-resistant nontyphoidal .
未分型 （NTS） 是人类非伤寒沙门氏菌病的主要病原体。本研究旨在分析 2022 年在中国江苏省镇江市检测到的 8 株 NTS 的流行病学特征和水平转移机制。从食源性腹泻门诊患者中采集粪便样本。用 Vitek 2 Compact 系统鉴定 NTS 分离株，并进行药敏试验。用 Illumina NovaSeq 平台和 Oxford Nanopore Technologies 平台对 NTS 分离株进行基因组测序。利用相关的开放存取资源预测 AMR 基因和移动遗传元件（MGEs）。从 153 份标本中分离出 8 株 NTS，其中最常见的血清型为 Typhimurium ST19。检出率最高的 AMR 基因是 AAC(6)-Iaa（10.5%），其次是 TEM-1（7.9%）、sul2（6.6%）和 tet(A)（5.3%）。在 8 株 NTS 的染色体上发现了 11 种携带 34 种 AMR 基因的 MGEs，包括 3 个抗性岛、6 个复合转座子（Tns）和 2 个整合子。在 8 株 NTS 中检测到 18 个携带 40 种 AMR 基因的质粒，包括 6 个可移动质粒、3 个可接合质粒和 9 个不可转移质粒，其中 7 个携带 10 个复合 Tns 和 3 个整合子。本研究从遗传角度为镇江市 NTS 耐药的防控提供了理论依据。
人类非伤寒沙门氏菌病是细菌性食源性疾病的常见原因之一，具有重大的社会和经济影响，特别是由侵袭性多药耐药非伤寒沙门氏菌引起的疾病，发病率和死亡率都很高。抗生素耐药性主要由耐药基因介导，移动遗传元件在捕获、积累和传播抗生素耐药基因方面发挥关键作用。因此，有必要研究非伤寒沙门氏菌的抗生素耐药基因的流行病学特征和水平转移机制，以防止多药耐药非伤寒沙门氏菌的传播。
