Embedding method for electron microscopy in biology.

Transmission electron microscopes were commercially available in some foreign countries in the later 1930s. Sectioning for light microscopy was used for electron microscopic observations of the internal structure of cells and tissues in the early days. Paraffin waxes were tested as embedding media, but they were too soft to enable thin sections. Pease and Baker (1948) (74) achieved an early success with the double-embedding method using the plastic "Parlodion" and paraffin wax.