通过逆向运行红细胞钙泵实现净ATP合成。
Net ATP synthesis by running the red cell calcium pump backwards.
作者信息
Wüthrich A, Schatzmann H J, Romero P
出版信息
Experientia. 1979 Dec 15;35(12):1589-90. doi: 10.1007/BF01953210.
PMID:391586
Abstract
Ca2+ loaded inside-out vesicles from human red blood cells, yielding C2+ into a Ca2+ free medium with 4 mM EGTA, 2 mM ADP and 10 mM phosphate, produced an excess of 14.9 pmoles . min-1 . (mg protein)-1 of ATP compared to controls in which the transmembrane Ca2+ gradient was abolished by the ionophore A 23 187.
摘要
将人红细胞的内翻外囊泡加载Ca2+，使其在含有4 mM乙二醇双四乙酸（EGTA）、2 mM二磷酸腺苷（ADP）和10 mM磷酸盐的无Ca2+培养基中释放Ca2+，与通过离子载体A 23187消除跨膜Ca2+梯度的对照组相比，产生了过量的14.9皮摩尔·分钟-1·（毫克蛋白质）-1的三磷酸腺苷（ATP）。
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