Center for Applied Biotechnology Studies, Department of Biological Science, College of Natural Sciences and Mathematics, California State University Fullerton, 800 N State College Blvd, Fullerton, CA, USA.
Área Biología Molecular, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina.
Sci Rep. 2024 Aug 19;14(1):19145. doi: 10.1038/s41598-024-70216-w.
Carbapenem-resistant Acinetobacter baumannii (CRAB) is a major human pathogen and a research priority for developing new antimicrobial agents. CRAB is a causative agent of a variety of infections in different body sites. One of the manifestations is catheter-associated urinary tract infection, which exposes the bacteria to the host's urine, creating a particular environment. Exposure of two CRAB clinical isolates, AB5075 and AMA40, to human urine (HU) resulted in the differential expression levels of 264 and 455 genes, respectively, of which 112 were common to both strains. Genes within this group play roles in metabolic pathways such as phenylacetic acid (PAA) catabolism, the Hut system, the tricarboxylic acid (TCA) cycle, and other processes like quorum sensing and biofilm formation. These results indicate that the presence of HU induces numerous adaptive changes in gene expression of the infecting bacteria. These changes presumably help bacteria establish and thrive in the hostile conditions in the urinary tract. These analyses advance our understanding of CRAB's metabolic adaptations to human fluids, as well as expand knowledge on bacterial responses to distinct human fluids containing different concentrations of human serum albumin (HSA).
耐碳青霉烯鲍曼不动杆菌（CRAB）是一种主要的人类病原体，也是开发新抗菌药物的研究重点。CRAB 是多种不同部位感染的病原体。其中一种表现形式是与导管相关的尿路感染，它使细菌暴露于宿主的尿液中，形成一个特殊的环境。将两种 CRAB 临床分离株 AB5075 和 AMA40 暴露于人尿（HU）中，分别导致 264 个和 455 个基因的表达水平出现差异，其中 112 个基因在两种菌株中都存在。该组内的基因在苯乙酸（PAA）代谢、Hut 系统、三羧酸（TCA）循环等代谢途径以及群体感应和生物膜形成等其他过程中发挥作用。这些结果表明，HU 的存在诱导了感染细菌基因表达的大量适应性变化。这些变化可能有助于细菌在尿路的恶劣环境中建立和繁殖。这些分析增进了我们对 CRAB 对人液代谢适应的理解，同时也扩展了关于细菌对含有不同浓度人血清白蛋白（HSA）的不同人液的反应的知识。
