Interdisciplinary Graduate School of Agriculture and Engineering, University of Miyazaki, Miyazaki, Japan.
Jin-Sing Chen's Mushroom Farm, No. 31, Donghu Road, Dali District, Taichung City, Taiwan, ROC.
Folia Microbiol (Praha). 2024 Oct;69(5):1137-1144. doi: 10.1007/s12223-024-01189-6. Epub 2024 Aug 19.
Sarcodon aspratus (Berk.) S. Ito is a Japanese local dish with unique aroma and is effective against allergic diseases. However, its cultivation was still difficult. Recently, coexisting bacteria were regarded as an important factor for mycelium growth and fruiting body formation. Therefore, we performed 16S rRNA amplicon sequencing in the fruiting body of S. aspratus and its adhered soil to understand the bacterial communities in the fruiting body of S. aspratus. The fruiting body group showed lower alpha diversities and a significant difference in the structure of bacterial communities compared to the soil group. In addition, Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium had the highest relative abundance in the fruiting body group, and it was also a potential coexisting bacterium in the fruiting body of S. aspratus by linear discriminant analysis effect size (LEfSe) analysis. This highest relative abundance phenomenon in Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium clade was also found in the fruiting body of Cantharellus cibarius. These findings suggested that Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium plays a key role in the bacterial communities in the fruiting body of S. aspratus. Bacteria in the fruit bodies of S. aspratus and C. cibarius probably present a similar coexistence model.
皱盖假芝（Berk.）S. Ito 是一种具有独特香气的日本地方菜肴，对过敏性疾病有效。然而，其栽培仍然困难。最近，共存细菌被认为是菌丝生长和子实体形成的重要因素。因此，我们对皱盖假芝及其附着土壤的子实体进行了 16S rRNA 扩增子测序，以了解皱盖假芝子实体中的细菌群落。与土壤组相比，子实体组的α多样性较低，细菌群落结构存在显著差异。此外，在子实体组中，Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium 的相对丰度最高，通过线性判别分析效应大小（LEfSe）分析，它也是皱盖假芝子实体中的潜在共存细菌。这种 Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium 分支的最高相对丰度现象也在 Cantharellus cibarius 的子实体中发现。这些发现表明，Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium 在皱盖假芝子实体的细菌群落中发挥着关键作用。皱盖假芝和鸡油菌子实体中的细菌可能呈现出相似的共存模式。
