在美国，在宿主寻找的肩突硬蜱和太平洋硬蜱中没有巴尔通体感染的证据。
No evidence of Bartonella infections in host-seeking Ixodes scapularis and Ixodes pacificus ticks in the United States.
机构信息
Division of Vector-Borne Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, USA.
US Department of Agriculture, National Wildlife Research Center, Wildlife Services, Animal and Plant Health Inspection Service, Fort Collins, CO, USA.
出版信息
Parasit Vectors. 2024 Aug 19;17(1):345. doi: 10.1186/s13071-024-06386-3.
BACKGROUND
Bartonella spp. infect a variety of vertebrates throughout the world, with generally high prevalence. Several Bartonella spp. are known to cause diverse clinical manifestations in humans and have been recognized as emerging pathogens. These bacteria are mainly transmitted by blood-sucking arthropods, such as fleas and lice. The role of ticks in the transmission of Bartonella spp. is unclear.
METHODS
A recently developed quadruplex polymerase chain reaction (PCR) amplicon next-generation sequencing approach that targets Bartonella-specific fragments on gltA, ssrA, rpoB, and groEL was applied to test host-seeking Ixodes scapularis ticks (n = 1641; consisting of 886 nymphs and 755 adults) collected in 23 states of the eastern half of the United States and Ixodes pacificus ticks (n = 966; all nymphs) collected in California in the western United States for the presence of Bartonella DNA. These species were selected because they are common human biters and serve as vectors of pathogens causing the greatest number of vector-borne diseases in the United States.
RESULTS
No Bartonella DNA was detected in any of the ticks tested by any target.
CONCLUSIONS
Owing to the lack of Bartonella detection in a large number of host-seeking Ixodes spp. ticks tested across a broad geographical region, our results strongly suggest that I. scapularis and I. pacificus are unlikely to contribute more than minimally, if at all, to the transmission of Bartonella spp.
背景
巴尔通体属（Bartonella spp.）在世界各地的各种脊椎动物中感染，普遍流行率较高。有几种巴尔通体属已知会引起人类多种临床表现，并已被确认为新兴病原体。这些细菌主要通过吸血节肢动物传播，如跳蚤和虱子。蜱在巴尔通体属传播中的作用尚不清楚。
方法
采用一种新开发的四重聚合酶链反应（PCR）扩增子下一代测序方法，该方法针对 gltA、ssrA、rpoB 和 groEL 上的巴尔通体特异性片段，用于检测在美国东半部 23 个州采集的宿主寻找的肩突硬蜱（Ixodes scapularis）（n=1641；包括 886 只若虫和 755 只成虫）和在美国西部加利福尼亚州采集的太平洋硬蜱（Ixodes pacificus）（n=966；均为若虫）中是否存在巴尔通体 DNA。选择这些物种是因为它们是常见的人类叮咬者，并且是导致美国数量最多的虫媒病的病原体的传播媒介。
结果
在所检测的任何目标中，都没有检测到任何蜱虫的巴尔通体 DNA。
结论
由于在广泛的地理区域内对大量宿主寻找的硬蜱属蜱虫进行检测均未发现巴尔通体，因此我们的结果强烈表明，肩突硬蜱和太平洋硬蜱不太可能对巴尔通体属的传播有较大的贡献，如果有的话。
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