Center for Infection and Immunity, Mailman School of Public Health, Columbia University, NY, United States.
Center for Infection and Immunity, Mailman School of Public Health, Columbia University, NY, United States.
Ticks Tick Borne Dis. 2019 Jun;10(4):894-900. doi: 10.1016/j.ttbdis.2019.04.011. Epub 2019 Apr 15.
We employed high throughput sequencing to survey the microbiomes of Ixodes scapularis collected in New York and Connecticut. We examined 197 individual I. scapularis adults and pools from 132 adults and 197 nymphs. We detected Borrelia burgdorferi sensu stricto in 56.3% of individual ticks, Anaplasma phagocytophilum in 10.6%, Borrelia miyamotoi in 5%, Babesia microti in 7.6%, and Powassan virus in 3.6%. We did not detect Borrelia mayonii, Ehrlichia muris eauclairensis, Bartonella spp. or pathogenic Babesia species other than B. microti. The most abundant bacterium (65%), and only rickettsial species identified, was the endosymbiont Rickettsia buchneri. A filarial nematode was found in 13.7% of adult ticks. Fourteen viruses were detected including South Bay virus (22%) and blacklegged tick phlebovirus 1 and 2 (73%). This study provides insight into the microbial diversity of I. scapularis in New York State and Connecticut.
我们采用高通量测序技术调查了在纽约和康涅狄格州采集的硬蜱的微生物组。我们检查了 197 只成年硬蜱和 132 只成蜱和 197 只若蜱的样本。我们在 56.3%的个体蜱中检测到了伯氏疏螺旋体，10.6%的个体蜱中检测到了嗜吞噬细胞无形体，5%的个体蜱中检测到了伯氏疏螺旋体，7.6%的个体蜱中检测到了微小巴贝斯虫，3.6%的个体蜱中检测到了波瓦桑病毒。我们没有检测到梅奥尼亚莱姆病螺旋体、埃氏鼠埃立克体、巴尔通体属或除微小巴贝斯虫以外的其他致病性巴贝斯虫。最丰富的细菌（65%）和唯一鉴定出的立克次体是内共生菌 Rickettsia buchneri。在 13.7%的成年蜱中发现了一种丝虫。共检测到 14 种病毒，包括南湾病毒（22%）和黑腿蜱病毒 1 和 2（73%）。本研究为了解纽约州和康涅狄格州硬蜱的微生物多样性提供了线索。
