增强咀嚼力对去卵巢大鼠牙槽骨中 OPG、RANKL 和 MGF 的影响。
Effect of enhanced masticatory force on OPG, RANKL and MGF in alveolar bone of ovariectomized rats.
机构信息
Dalian University, Mechanical Engineering College, Dalian, China.
Dalian University, Graduate School, Dalian, China.
出版信息
J Appl Oral Sci. 2020 Apr 3;28:e20190409. doi: 10.1590/1678-7757-2019-0409. eCollection 2020.
BACKGROUND
Menopause induces oral bone loss, leading to various oral diseases. Mastication importantly affects bone metabolism in the jawbone.
OBJECTIVE
To analyze the effect of enhanced masticatory force on osteoprotegerin (OPG), receptor activator of nuclear factor kappa B ligand (RANKL), and mechano-growth factor (MGF) in alveolar bone of ovariectomized rats and to study the mechanics mechanism of the alveolar bone of ovariectomized rats response to enhanced masticatory force.
METHODOLOGY
Thirty Sprague Dawley rats were randomly divided into three groups: sham-operation group (fat around the removed ovary + normal hard diet), model group (ovariectomy + normal hard diet), and experimental group (ovariectomy + high hard diet). It was a 2-month experiment. Enzyme-linked immunosorbent assay (ELISA) detected serum estradiol (E2), osteocalcin (BGP) and alkaline phosphatase (ALP) in rats. Bone histomorphometric indices in the third molar region of maxilla were detected by micro-CT; protein expressions of OPG, RANKL, and MGF in the third molar region of maxilla was detected by Western blot; and gene expression of OPG, RANKL, and MGF in the third molar region of maxilla was detected by Quantitative Real-Time PCR.
RESULTS
Comparing with model group, serum E2 in experimental group increased but not significantly, serum BGP and serum ALP in experimental group decreased but not significantly, OPG in experimental group in alveolar bone increased significantly, RANKL in experimental group in alveolar bone decreased significantly, RANKL/OPG ratio in experimental group decreased significantly, MGF in experimental group in alveolar bone increased significantly, bone volume to total volume fraction increased significantly in experimental group, trabecular thickness increased significantly in experimental group, and trabecular separation decreased significantly in experimental group.
CONCLUSION
Enhanced masticatory force affected the expression of OPG, RANKL, and MGF in alveolar bone of ovariectomized rats, improved the quality of jaw bone of ovariectomized rats, and delayed oral bone loss by ovariectomy.
背景
绝经会导致口腔骨质流失，进而引发各种口腔疾病。咀嚼对颌骨中的骨代谢有重要影响。
目的
分析增强咀嚼力对去卵巢大鼠牙槽骨中骨保护素（OPG）、核因子κB 受体激活剂配体（RANKL）和机械生长因子（MGF）的影响，并研究去卵巢大鼠牙槽骨对增强咀嚼力的反应的力学机制。
方法
30 只 Sprague Dawley 大鼠随机分为 3 组：假手术组（去除卵巢周围脂肪+正常硬食）、模型组（卵巢切除术+正常硬食）和实验组（卵巢切除术+高硬食）。实验持续 2 个月。酶联免疫吸附试验（ELISA）检测大鼠血清雌二醇（E2）、骨钙素（BGP）和碱性磷酸酶（ALP）。通过 micro-CT 检测上颌第三磨牙区的骨组织形态计量学指标；通过 Western blot 检测上颌第三磨牙区 OPG、RANKL 和 MGF 的蛋白表达；通过定量实时 PCR 检测上颌第三磨牙区 OPG、RANKL 和 MGF 的基因表达。
结果
与模型组相比，实验组血清 E2 略有增加但不显著，血清 BGP 和血清 ALP 略有减少但不显著，实验组牙槽骨 OPG 明显增加，牙槽骨 RANKL 明显减少，RANKL/OPG 比值明显降低，实验组牙槽骨 MGF 明显增加，实验组骨体积与总体积比明显增加，实验组骨小梁厚度明显增加，实验组骨小梁分离度明显降低。
结论
增强咀嚼力影响去卵巢大鼠牙槽骨中 OPG、RANKL 和 MGF 的表达，改善去卵巢大鼠颌骨质量，延缓卵巢切除引起的口腔骨丢失。
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