Wang Jingyi, Huang Hu, Tao Kan, Guo Lili, Hu Xincheng, Chang Huailong
Global R&D Center, Shanghai Chicmax Cosmetic Co. Ltd., Global Harbor Tower B, Shanghai, China.
J Cosmet Dermatol. 2024 Dec;23(12):4334-4342. doi: 10.1111/jocd.16531. Epub 2024 Aug 19.
Skin aging is one of the most abundant aging-related disorders that can be accelerated by excessive exposure to ultraviolet irradiation. Topically applied fermented skincare ingredients have gained mounting attentions due to their high concentration of various skin nourishing nutrients and bioactive components and low skin irritation potency.
In the present study, we aim to fully demonstrate the skin-related benefits of a novel extract of Thermus thermophilus and Bacillus subtilis mixed-culture ferment (TBFE).
TBFE was prepared through an innovative mixed-culture fermentation process. The contents of nutrients and bioactive ingredients were quantified by different methods accordingly. Both in vitro tests and randomized controlled human trial were utilized to further demonstrate multifaceted beneficial effects on human skin, as well as the potential mechanisms.
Our results showed that TBFE upregulated the expression of type IV collagen, elastin, aquaporin-3, and dermal-epidermal junction markers, while inhibited production of melanin, in different skin cell models. Moreover, TBFE inhibited the generation of reactive oxygen species and pro-inflammatory mediators induced by ultraviolet irradiation in normal human keratinocytes, while stimulated autophagy in senescent keratinocytes. Results from clinical studies confirmed those in vitro findings, demonstrating that TBFE at 5% and 20% concentration provides anti-aging properties in subjects with sensitive skin, in terms of improving wrinkles, moisturization, and skin lightening.
In summary, we demonstrate that a novel mixed-culture ferment extract has promising anti-aging effects, which may be attributed to anti-oxidation, anti-inflammation, and promotion of autophagy in skin cells.
皮肤老化是最常见的与衰老相关的病症之一，过度暴露于紫外线照射会加速皮肤老化。局部应用的发酵护肤成分因其含有高浓度的各种皮肤滋养营养物质和生物活性成分以及低皮肤刺激性而受到越来越多的关注。
在本研究中，我们旨在充分证明嗜热栖热菌和枯草芽孢杆菌混合培养发酵的新型提取物（TBFE）对皮肤的益处。
通过创新的混合培养发酵工艺制备TBFE。相应地采用不同方法对营养成分和生物活性成分的含量进行定量。利用体外试验和随机对照人体试验进一步证明其对人体皮肤的多方面有益作用及其潜在机制。
我们的结果表明，在不同的皮肤细胞模型中，TBFE上调了IV型胶原蛋白、弹性蛋白、水通道蛋白-3和真皮-表皮连接标志物的表达，同时抑制了黑色素的产生。此外，TBFE抑制正常人角质形成细胞中紫外线照射诱导的活性氧和促炎介质的产生，同时刺激衰老角质形成细胞中的自噬。临床研究结果证实了体外研究结果，表明5%和20%浓度的TBFE在改善皱纹、保湿和提亮肤色方面为敏感性皮肤受试者提供抗老化特性。
总之，我们证明了一种新型混合培养发酵提取物具有有前景的抗老化作用，这可能归因于其在皮肤细胞中的抗氧化、抗炎和促进自噬作用。
