Protective effect of Cornus walteri Wangerin leaf against UVB irradiation induced photoaging in human reconstituted skin.

ETHNOPHARMACOLOGICAL RELEVANCE

Cornus walteri Wangerin has been used in oriental traditional medicine for the treatment of antidiarrheal and inflammation.

AIM OF THE STUDY

The efficacy of Cornus walteri Wangerin on skin anti-photoaging was investigated.

MATERIALS AND METHODS

Hydrolyzed Cornus walteri Wangerin leaf was tested for the anti-photoaging effects against ultraviolet B (UVB)-induced matrix metalloproteinase (MMP)-1, pro-inflammatory cytokines using human reconstituted skin (KeraSkin™-FT) and also tested for elastase activity in vitro. The MMP-1 and pro-inflammatory cytokine levels of the extract were evaluated by enzyme-linked immunosorbent assay (ELISA).

RESULTS

The extract of hydrolyzed Cornus walteri Wangerin leaf (CWE) had the elastase inhibitory activity (IC: 0.457mg/mL). CWE inhibited MMP-1 expression up to 61% in comparison with the control group which was not treated using CWE, but exposed to UVB. CWE also showed an inhibitory effect on releasing pro-inflammatory cytokines (IL-6 and IL-8) in KeraSkin™-FT (30% and 57% inhibition at dose of 50μg/mL, respectively).

CONCLUSION

CWE is a promising anti-photoaging agent for the treatment of UVB-induced skin.