Ebrahimzadeh Manijeh, Asgharpour Fariba, Shokri Shirvani Javad, Kazemi Sohrab, Moghadamnia Ali Akbar
Student Research Committee Health Research Institute Babol University of Medical Sciences, Babol, Iran.
Department of Laboratory Sciences Faculty of Para-Medicine Babol University of Medical Sciences, Babol, Iran.
Adv Pharmacol Pharm Sci. 2024 Aug 12;2024:6380155. doi: 10.1155/2024/6380155. eCollection 2024.
(), a widespread bacterial pathogen, is associated with various gastrointestinal diseases, including gastric cancer. Statins, widely prescribed cholesterol-lowering agents, have demonstrated pleiotropic effects, including potential antimicrobial properties. This study investigated the direct antibacterial effects of three clinically approved statins, simvastatin, atorvastatin, and rosuvastatin, against isolates.
strains were isolated from gastric biopsies of dyspeptic patients and identified by microbiological techniques. The minimum inhibitory concentrations (MICs) of statins were determined using the agar dilution method, and their antimicrobial activity was evaluated by the disc diffusion method using different concentrations of simvastatin, atorvastatin, rosuvastatin, tetracycline, and amoxicillin. Scanning electron microscopy (SEM) was employed to examine the morphology of cells.
The minimum inhibitory concentration (MIC) values (g/mL) of atorvastatin, rosuvastatin, simvastatin, tetracycline, and amoxicillin against were 240 ± 20, 450 ± 20, 460 ± 15, 155 ± 30, and 140 ± 20, respectively. In the disc diffusion assay, atorvastatin and rosuvastatin produced significantly larger inhibition zones compared to simvastatin at all concentrations tested ( < 0.05). The inhibition zone diameters (mm) increased with higher statin concentrations, ranging from 9 ± 1.4 to 13 ± 1.4 for atorvastatin, 8 ± 0.9 to 11 ± 0.6 for rosuvastatin, and 5 ± 1.3 to 6 ± 1.4 for simvastatin at the highest tested concentration (1200 g/ml). SEM analysis revealed the characteristic spiral morphology of cells.
Statins demonstrated varying degrees of antibacterial activity against isolates, with atorvastatin exhibiting the highest potency. While the observed effects were lower than those of conventional antibiotics, these findings suggest the potential of statins as adjunctive agents or alternative therapeutic options, warranting further investigation through studies and clinical trials.
（）是一种广泛传播的细菌病原体，与包括胃癌在内的多种胃肠道疾病有关。他汀类药物是广泛使用的降胆固醇药物，已显示出多效性作用，包括潜在的抗菌特性。本研究调查了三种临床批准的他汀类药物，辛伐他汀、阿托伐他汀和瑞舒伐他汀，对分离株的直接抗菌作用。
从消化不良患者的胃活检组织中分离出菌株，并通过微生物学技术进行鉴定。使用琼脂稀释法测定他汀类药物的最低抑菌浓度（MIC），并使用不同浓度的辛伐他汀、阿托伐他汀、瑞舒伐他汀、四环素和阿莫西林，通过纸片扩散法评估它们的抗菌活性。采用扫描电子显微镜（SEM）检查细胞的形态。
阿托伐他汀、瑞舒伐他汀、辛伐他汀、四环素和阿莫西林对的最低抑菌浓度（MIC）值（μg/mL）分别为240±20、450±20、460±15、155±30和140±20。在纸片扩散试验中，在所有测试浓度下，阿托伐他汀和瑞舒伐他汀产生的抑菌圈明显大于辛伐他汀（P<0.05）。抑菌圈直径（mm）随着他汀类药物浓度的升高而增加，在最高测试浓度（1200μg/ml）下，阿托伐他汀的抑菌圈直径从9±1.4增加到13±1.4，瑞舒伐他汀从8±0.9增加到11±0.6，辛伐他汀从5±1.3增加到6±1.4。SEM分析显示了细胞的特征性螺旋形态。
他汀类药物对分离株表现出不同程度的抗菌活性，阿托伐他汀的效力最高。虽然观察到的效果低于传统抗生素，但这些发现表明他汀类药物作为辅助剂或替代治疗选择的潜力，值得通过进一步的研究和临床试验进行深入调查。
