Seiler Christian, Luepke Matthias, Bach Jan-Peter, Seifert Hermann
Institute of General Radiology and Medical Physics, University of Veterinary Medicine Foundation, Hannover, Germany.
Small Animal Clinic, University of Veterinary Medicine Foundation, Hannover, Germany.
Vet Med (Praha). 2022 May 1;67(7):387-394. doi: 10.17221/100/2020-VETMED. eCollection 2022 Jul.
The non-invasive determination of the vasculature volume would be very useful in many fields of medicine such as oncology and implantation. The purpose of this research was, therefore, to develop a methodology to investigate vascularisation in phantoms using microcomputed tomography (μCT) without having to visualise the single vessels. Epoxy resin and cotton candy were used to form the phantoms with microchannels. The size of the channels was measured via microscopy and the proportion of the void volume (PVV) was calculated. The phantoms were placed in contrast agent solutions of different concentrations and scanned in μCT. The mean CT numbers of the phantoms were calculated with the Amira software and displayed as a function of the determined PVV and the contrast agent concentration (CAC). The fabricated microchannels had the size of biological capillaries (diameter: 5 μm to 15 μm) and the phantoms showed a microchannel density of 5 to15 microchannels per mm². With an increasing CAC, the CT numbers increased significantly. Additionally, the phantoms with a higher PVV also had a higher CT number. The CT numbers and the PVV correlated moderately together, but significantly. The slope of the regression line increased with an increasing CAC.
血管系统容积的非侵入性测定在肿瘤学和植入等许多医学领域将非常有用。因此，本研究的目的是开发一种方法，利用微型计算机断层扫描（μCT）研究体模中的血管生成，而无需可视化单个血管。环氧树脂和棉花糖被用于制作带有微通道的体模。通过显微镜测量通道尺寸，并计算孔隙体积比例（PVV）。将体模置于不同浓度的造影剂溶液中，并在μCT中进行扫描。使用Amira软件计算体模的平均CT值，并将其显示为所测定的PVV和造影剂浓度（CAC）的函数。制作的微通道尺寸与生物毛细血管相当（直径：5μm至15μm），体模的微通道密度为每平方毫米5至15个微通道。随着CAC的增加，CT值显著增加。此外，PVV较高的体模CT值也较高。CT值与PVV呈中度但显著的相关性。回归线的斜率随着CAC的增加而增大。
